REBASE-The Restriction Enzyme Database

REBASE-限制性内切酶数据库

• 批准号: 7897197
• 负责人: RICHARD J ROBERTS
• 金额: $ 25万
• 依托单位: NEW ENGLAND BIOLABS, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7897197
• 关键词: Biotechnology; Cleaved cell; Community Healthcare; DNA; DNA Restriction Enzymes; Data; Databases; Electronic Mail; Electronics; GCG gene; Health Services Research; Information Dissemination; Internet; Literature; Maintenance; Methyltransferase; Microbial Genome Sequencing; New England; Publications; Publishing; Research Personnel; Resources; Restriction Mapping; Site; Specificity; Update; computer program; formycin triphosphate; methylcobalamin-coenzyme M methyltransferase; performance site; research study; restriction enzyme

DESCRIPTION (provided by applicant): This project concerns the maintenance and distribution of a cohesive and comprehensive database of information about restriction endonucleases and their associated methyltransferases. Key components of the database are information about the methyl sensitivity of restriction endonucleases and a comprehensive analysis of the restriction enzymes present in the sequenced microbial genomes. The performance site is New England Biolabs, located in Beverly, MA. REBASE has been in existence since 1975 and is currently implemented in a relational format in SYBASE. Information enters the database both from the published literature and by direct submission from the investigators either prior to publication or instead of publication. Approximately 10% of the entries describing experimental data are unpublished other than through REBASE. The information in REBASE is disseminated throughout the world both by formal publication in the scientific literature and in a variety of electronic formats. We post monthly updates and selected files are distributed through an automatic email list to more than 400 sites. In addition, the information is available by anonymous FTP or through the worldwide web. Among the files maintained and updated monthly, are specialized ones that are used by many of the more widely used computer programs such as GCG, McVector, DNA Strider, LaserGene, etc. These files are ready to use and include up-to-date information about the full range of restriction endonuclease specificities and their commercial availabilities. Information available through REBASE is key to all investigators planning experiments to cleave and/or religate DNA. It is a unique resource for the practice of biotechnology and its contents are used directly or indirectly by many sections of the research health care community.

描述（由申请人提供）： 该项目涉及有关限制性核酸内切酶及其相关甲基转移酶的凝聚力和全面数据库的维护和分布。数据库的关键组成部分是有关限制性核酸内切酶的甲基敏感性的信息，以及对测序微生物基因组中存在的限制酶的全面分析。表演地点是位于马萨诸塞州比佛利的新英格兰Biolabs。 自1975年以来，rebase一直存在，目前以关系格式实施 Sybase。信息从已发表的文献和调查人员直接提交之前，都可以在发布之前或而不是出版。描述实验数据的条目中约有10％是未发表的。信息中的信息 重生是通过科学文献和各种电子格式的正式出版物在世界范围内传播的。我们发布每月更新，选定的文件通过自动电子邮件列表分配给400多个站点。此外，该信息可通过匿名FTP或通过全球网络获得。 在每月维护和更新的文件中，有许多专门的文件。 使用更广泛使用的计算机程序，例如GCG，McVector，DNA Strider，Lasergene等。这些文件已准备好使用，并包含有关限制性内核酶的全部最新信息 特殊性及其商业可用性。通过rebase获得的信息是所有人的关键 调查人员计划裂解和/或宗教DNA实验。它是生物技术实践的独特资源，其含量直接或间接地由研究医疗保健社区的许多部分使用。