REBASE-The Restriction Enzyme Database

REBASE-限制性内切酶数据库

• 批准号: 7897197
• 负责人: RICHARD J ROBERTS
• 金额: $ 25万
• 依托单位: NEW ENGLAND BIOLABS, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7897197
• 关键词: Biotechnology; Cleaved cell; Community Healthcare; DNA; DNA Restriction Enzymes; Data; Databases; Electronic Mail; Electronics; GCG gene; Health Services Research; Information Dissemination; Internet; Literature; Maintenance; Methyltransferase; Microbial Genome Sequencing; New England; Publications; Publishing; Research Personnel; Resources; Restriction Mapping; Site; Specificity; Update; computer program; formycin triphosphate; methylcobalamin-coenzyme M methyltransferase; performance site; research study; restriction enzyme

DESCRIPTION (provided by applicant): This project concerns the maintenance and distribution of a cohesive and comprehensive database of information about restriction endonucleases and their associated methyltransferases. Key components of the database are information about the methyl sensitivity of restriction endonucleases and a comprehensive analysis of the restriction enzymes present in the sequenced microbial genomes. The performance site is New England Biolabs, located in Beverly, MA. REBASE has been in existence since 1975 and is currently implemented in a relational format in SYBASE. Information enters the database both from the published literature and by direct submission from the investigators either prior to publication or instead of publication. Approximately 10% of the entries describing experimental data are unpublished other than through REBASE. The information in REBASE is disseminated throughout the world both by formal publication in the scientific literature and in a variety of electronic formats. We post monthly updates and selected files are distributed through an automatic email list to more than 400 sites. In addition, the information is available by anonymous FTP or through the worldwide web. Among the files maintained and updated monthly, are specialized ones that are used by many of the more widely used computer programs such as GCG, McVector, DNA Strider, LaserGene, etc. These files are ready to use and include up-to-date information about the full range of restriction endonuclease specificities and their commercial availabilities. Information available through REBASE is key to all investigators planning experiments to cleave and/or religate DNA. It is a unique resource for the practice of biotechnology and its contents are used directly or indirectly by many sections of the research health care community.

描述(由申请人提供)： 该项目涉及维持和分配一个关于限制性内切酶及其相关甲基转移酶信息的连贯和全面的数据库。该数据库的关键组成部分是关于限制性内切酶的甲基敏感性的信息，以及对已测序微生物基因组中存在的限制性内切酶的全面分析。演出地点是位于马萨诸塞州贝弗利的新英格兰Biolabs。 Rebase自1975年就已存在，目前以关系格式在 赛贝斯。信息既来自已发表的文献，也通过调查人员在出版前或代替出版前直接提交的方式进入数据库。描述实验数据的条目中，约有10%是通过Rebase以外的方式未发布的。中的信息 REBASE通过正式出版的科学文献和各种电子格式在世界各地传播。我们每月发布更新，选定的文件通过自动电子邮件列表分发到400多个站点。此外，这些信息还可以通过匿名的ftp或通过万维网获得。 在每月维护和更新的文件中，有专门的文件，许多 更广泛使用的计算机程序，如GCG、McVector、DNA Strider、Lasergene等。这些文件随时可用，并包括有关所有限制性内切酶的最新信息 特性和它们的商业可用性。通过Rebase获得的信息是所有人的关键 研究人员计划进行实验，以切割和/或信仰DNA。它是生物技术实践的独特资源，其内容被研究保健界的许多部门直接或间接使用。