ENGINEERING THE VOCAL FOLD EXTRACELLULAR MATRIX

声带细胞外基质的工程设计

• 批准号: 6497894
• 负责人: STEVEN D GRAY
• 金额: $ 56.19万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2005-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497894
• 关键词: bioengineering /biomedical engineering; biomechanics; extracellular matrix; fibroblasts; goats; hyaluronate; laboratory rat; physiologic stressor; speech therapy; tissue support frame; vibration; vocal cords

Vocal cord oscillation is governed by the biomechanical properties of the tissue. These biomechanical properties, such as viscosity and elasticity are inherent in the extracellular matrix (ECM) of the vocal fold lamina propria. The composition of the ECM is controlled mainly by the fibroblast. Current theories support factors, and forces felt by the fibroblast. Management of the ECM may be accomplished by manipulation of the factors that effect the fibroblast, or directly changing the CM with injectable or implantable agents or materials. This proposal investigates the effect of forces on the fibroblast and ECM, selects agents for injection into the ECM that optimally influence the biomechanics of the vocal fold, and lays the groundwork for creating implantable, artificial matrices with favorable ECM properties for vocal tissue oscillation. Vocal tissue engineering, in a broad sense, includes, many possible methods that ultimately lead to tissues that has been preticatble created or influenced for a designated functional purpose. This proposal investigates areas that will potentially lead to three future clinical treatments that may predictably manage the biomechanical properties of the vocal flood by changing the composition of the ECM or replacing the ECM. These eventual areas are: 1) specific speech therapy exercises designed to expose the fibroblast to forces that lead to a remodeling of the ECM; 2) infections to manipulate the fibroblast and/or ECM, and 4) combinations of these.

声带振荡由组织的生物力学特性控制。这些生物力学特性，例如粘度和弹性，是声带固有层的细胞外基质 (ECM) 所固有的。 ECM的组成主要由成纤维细胞控制。当前的理论支持成纤维细胞感受到的因素和力量。 ECM的管理可以通过操纵影响成纤维细胞的因素来完成，或者用可注射或可植入的试剂或材料直接改变CM。该提案研究了力对成纤维细胞和 ECM 的影响，选择注射到 ECM 中的药剂，以最佳地影响声带的生物力学，并为创建具有良好 ECM 特性的声带组织振荡的可植入人工基质奠定了基础。从广义上讲，声音组织工程包括许多可能的方法，这些方法最终导致为指定的功能目的而创建或影响的组织。该提案研究了可能导致三种未来临床治疗的领域，这些治疗可以通过改变 ECM 的成分或更换 ECM 来可预测地管理声乐洪水的生物力学特性。这些最终领域是：1）特定的言语治疗练习，旨在使成纤维细胞暴露于导致 ECM 重塑的力量； 2) 操纵成纤维细胞和/或 ECM 的感染，以及 4) 这些的组合。