BASEMENT MEMBRANE ZONE IN VOCAL INJURY

这个项目研究的是表皮-孔板连接， 被称为基底膜带(BMZ)。基本问题 是什么将表皮保持在纤维结构上？ 固有层？“和”为什么良性病变如此常见？“ 喉部疾病？人类声带小结和Reinke‘s的组织学 水肿提示BMZ在声带损伤中起重要作用。 皮肤中的BMZ由附着结构组成，如 帮助固定椎板的半桥粒和锚定纤维 从固有层到表皮。皮肤中的BMZ也含有许多 帮助保持或“粘合”BMZ完整的蛋白质。 通过电子显微镜，将进行形态测量研究 确定附着的BMZ结构的人口密度 在正常的人和狗的声带中。因为皮肤病 研究表明，依恋的人口密度存在差异 跨个人的结构，项目将确定正常 附着BMZ结构的范围。通过免疫荧光， 和免疫电子显微镜技术，BMZ的蛋白质 将在正常的人类脐带中表现出来。在规范数据之后 已经获得，它将被用于帮助确定异常 在受伤的脐带中。 人们怀疑声带小结是BMZZ损伤的结果。 结构。声带小结、息肉或Reinke‘s水肿会 被检查以确定a)某些个体是否固有地 具有少量的附着结构或蛋白质，从而导致 发声损伤的阈值较低，以及b)慢性损伤 这些结构导致了BMZ的生物学变化。 此外，发声损伤的急性犬模型将 检查是否有BMZ损伤。 调查将确定这两个架构(使用 电子显微镜)和生物成分(使用 免疫荧光技术)。声乐病机 对损伤的研究将与可能的解剖学兴趣一起进行 可能对部分声带造成损伤的因素。 这是一个长期的计划，以获得更多的技能在国家 ART电子显微镜技术与免疫荧光 喉部解剖学和生物学研究技术 组件。有了这些技能，良性喉部疾病会导致 发声功能障碍和治疗将进行研究。

This project investigates the epidermal-lamina poropria junction, knwon as the basement membrane zone (BMZ). The basic questions are, "What holds the epidermis to the fibrous structures of the lamina propria?" and "Why is BMZ pathology so frequent in benign laryngeal disease?" Histology of human vocal nodules and Reinke's edema suggests the BMZ plays an important role in vocal injury. The BMZ in skin is composed of attaching stuctures, such as hemidesmosomes and anchoring fibrils which help secure the lamina propria to the epidermis. The BMZ in skin also contains many proteins which help hold or "glue" the BMZ intact. Through electorn microscopy, morphometric studies will be performed to determine the population density of the attaching BMZ structures in normal human and canine vocal cords. Since dermatiological studies have shown variation in population densities of attaching structures across individuals, the project will determine normal ranges for attaching BMZ structures. Through immunofluorescent, and immunoelectron microscopic techniques, the proteins of the BMZ will be characterized in normal human cords. After normative data has been obtained, it will be used to help determine abnormalities of the BMZ in injured cords. It is suspected that vocal nodules are the result of injury to BMZZ structures. Vocal nodules, polyps or Reinke's edema specimens will be examined to determine if a) certain individuals may inherently have a low number of attaching structures or proteins leading to a lower threshold for phonatory injury and b) chronic injury to these structures lead to biological changes in the BMZ. Additionally, acute canine models of phonatory injury will be examined for BMZ injury. The investigation will identify both the architecture (using electron microscopy) and the biological composition (using immunoflourescence techniques) of the BMZ. Pathogenesis of vocal injury will be studied witb interest towards possible anatomical factors which may impose some vocal cords to injury. It is the long range plan to acquire greater skills in state of the art electron microscopic techniques and immunofluorescent techniques to investigate laryngeal anatomic and biological components. With these skills, benign laryngeal disease causing phonatory dysfunction and therapy will be studied.