BASEMENT MEMBRANE ZONE IN VOCAL INJURY

该项目研究表皮-孔板层交界处， 称为基底膜区 (BMZ)。 基本问题 是，“是什么将表皮固定在皮肤的纤维结构上？ 固有层？”以及“为什么 BMZ 病理学在良性肿瘤中如此常见” 喉部疾病？” 人类声带小结和 Reinke 的组织学 水肿表明 BMZ 在声带损伤中起着重要作用。 皮肤中的 BMZ 由附着结构组成，例如 半桥粒和锚定原纤维有助于固定椎板 固有层至表皮。 皮肤中的BMZ还含有许多 帮助保持或“粘合” BMZ 完整的蛋白质。 通过电子显微镜，将进行形态测量研究 确定附属 BMZ 结构的人口密度 在正常人类和犬科动物的声带中。 由于皮肤科 研究表明，依附群体的人口密度存在差异 跨个人的结构，该项目将确定正常 连接 BMZ 结构的范围。 通过免疫荧光， 和免疫电子显微镜技术，BMZ 的蛋白质 将在正常人类脐带中表征。 规范数据后 已获得，将用于帮助确定异常情况 受伤绳索中的 BMZ。 怀疑声带小结是BMZZ受伤造成的 结构。 声带小结、息肉或赖因克水肿标本将 进行检查以确定是否 a) 某些个人可能天生 具有少量的附着结构或蛋白质导致 发声损伤的阈值较低，b) 慢性损伤 这些结构导致 BMZ 发生生物学变化。 此外，急性发声损伤犬模型将 检查 BMZ 损伤。 调查将确定这两种架构（使用 电子显微镜）和生物成分（使用 BMZ 的免疫荧光技术）。 发声的发病机制 损伤将随着对可能的解剖学的兴趣进行研究 可能造成某些声带损伤的因素。 这是在当前状况下获得更多技能的长期计划 艺术电子显微镜技术和免疫荧光 研究喉部解剖学和生物学的技术 组件。 有了这些技巧，良性喉病引起 将研究发声功能障碍和治疗。

This project investigates the epidermal-lamina poropria junction, knwon as the basement membrane zone (BMZ). The basic questions are, "What holds the epidermis to the fibrous structures of the lamina propria?" and "Why is BMZ pathology so frequent in benign laryngeal disease?" Histology of human vocal nodules and Reinke's edema suggests the BMZ plays an important role in vocal injury. The BMZ in skin is composed of attaching stuctures, such as hemidesmosomes and anchoring fibrils which help secure the lamina propria to the epidermis. The BMZ in skin also contains many proteins which help hold or "glue" the BMZ intact. Through electorn microscopy, morphometric studies will be performed to determine the population density of the attaching BMZ structures in normal human and canine vocal cords. Since dermatiological studies have shown variation in population densities of attaching structures across individuals, the project will determine normal ranges for attaching BMZ structures. Through immunofluorescent, and immunoelectron microscopic techniques, the proteins of the BMZ will be characterized in normal human cords. After normative data has been obtained, it will be used to help determine abnormalities of the BMZ in injured cords. It is suspected that vocal nodules are the result of injury to BMZZ structures. Vocal nodules, polyps or Reinke's edema specimens will be examined to determine if a) certain individuals may inherently have a low number of attaching structures or proteins leading to a lower threshold for phonatory injury and b) chronic injury to these structures lead to biological changes in the BMZ. Additionally, acute canine models of phonatory injury will be examined for BMZ injury. The investigation will identify both the architecture (using electron microscopy) and the biological composition (using immunoflourescence techniques) of the BMZ. Pathogenesis of vocal injury will be studied witb interest towards possible anatomical factors which may impose some vocal cords to injury. It is the long range plan to acquire greater skills in state of the art electron microscopic techniques and immunofluorescent techniques to investigate laryngeal anatomic and biological components. With these skills, benign laryngeal disease causing phonatory dysfunction and therapy will be studied.