BIOANALYTICAL APPLICATIONS IN CAPILLARY ELECTROPHORESIS

毛细管电泳中的生物分析应用

• 批准号: 6481212
• 负责人: FRANK A GOMEZ
• 金额: $ 7.33万
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6481212
• 关键词: analytical method; binding proteins; bioreactors; capillary electrophoresis; chemical binding; chemical reaction; chemical synthesis; human subject; immunologic assay /test; macromolecule; method development; molecular shape; peptide chemical synthesis; peptides; proteins; reagent /indicator; single cell analysis

The broad goals of this research are to further develop capillary electrophoresis (CE) as a technique to study in-capillary microreactions and to study biomolecular non-covalent interactions. Its focus is on demonstrating principles and on developing useful bioanalytical procedures that can be applied to the analysis of enzyme kinetics and receptor-ligand interactions. Approaches will include in-capillary enzyme-catalyzed microreactions involving both single cell and non- single cell analysis using laser-induced fluorescence (LIF) and UV VIS detection, and affinity capillary electrophoresis (ACE). The research will examine enzymes and small biomolecules that are important in the development of disease and, hence, to the issue of public health. This understanding will strengthen the scientific base underlying the design, preparation, and application of studied. This research effort will provide the technician and graduate student technicians a solid background in chemistry by examining problems at the interface of chemistry, chemistry-related fields, and medicine. The research projects planned for the technicians are aimed at demonstrating the interdisciplinary nature of CE and its importance as a new and exciting instrument in solving broad scientific problems. The specific objectives of the research are to: * Expand the CE-microreactor concept in the analysis of enzyme- catalyzed reactions. * Examine receptor-ligand interactions using affinity CE (ACE). * Study the physical biochemistry of derivatized proteins using CE. This research will: * Extend the usefulness of CE using microscale quantities in bioanalytical applications. * Extend the utility of CE in examining biomolecular non-covalent interactions. * Examine the changes in protein-ligand interactions using covalently modified proteins.

这项研究的主要目标是进一步发展毛细管电泳法(CE)作为一种研究毛细管内微反应和研究生物分子非共价相互作用的技术。它的重点是演示原理和开发可用于分析酶动力学和受体-配体相互作用的有用的生物分析方法。方法将包括毛细管内酶催化的微量反应，涉及使用激光诱导荧光(LIF)和紫外可见检测的单细胞和非单细胞分析，以及亲和毛细管电泳法(ACE)。这项研究将研究酶和小生物分子，它们在疾病的发展中非常重要，因此对公共卫生问题也很重要。这一理解将加强所研究的设计、准备和应用的科学基础。这项研究工作将通过研究化学、化学相关领域和医学之间的问题，为技术人员和研究生技术人员提供坚实的化学背景。为技术人员计划的研究项目旨在证明CE的跨学科性质及其作为解决广泛科学问题的一种新的、令人兴奋的工具的重要性。研究的具体目标是：*将CE-微反的概念扩展到酶催化反应的分析中。*使用亲和CE(ACE)检查受体-配体的相互作用。*用毛细管电泳法研究衍生蛋白质的物理生物化学。这项研究将：*扩大CE在生物分析应用中使用微量的有用性。*扩大毛细管电泳法在研究生物分子非共价相互作用中的效用。*使用共价修饰的蛋白质研究蛋白质-配体相互作用的变化。