CAPILLARY ELECTROPHORESIS--SYNTHESIS AND MOLECULAR RECOGNITION STUDIES

毛细管电泳--合成和分子识别研究

• 批准号: 6271538
• 负责人: FRANK A GOMEZ
• 金额: $ 13.37万
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6271538
• 关键词: binding proteins; bioreactors; capillary electrophoresis; chemical binding; chemical reaction; chemical synthesis; conformation; immunologic assay /test; method development; molecular shape; organometallic compounds; peptide chemical synthesis; peptides; protein structure; proteins; reagent /indicator

This research will develop capillary electrophoresis (CE) as a technique for studying the synthesis and molecular recognition properties of biomolecules in particular those label led with inorganic/organometallic reagents. The specific objectives of the research are to: * Evaluate the reactions between inorganic/organometallic reagents and peptides and proteins and other biomolecules. * Define the usefulness of using CE as a microreactor in which to monitor the reactions of biomolecules and inorganic/organometallic reagents and in enzyme-catalyzed reactions. * Develop new synthetic organometallic reagents and methods in which to label biomolecules. * Test the utility of CE to examine the physical-organic chemistry of modified proteins. * Examine the chemical reactivity of inorganic/organometallic-labelled biomolecules. * Explore applications of CE to binding assays and in non-aqueous and mixed solvent systems. * Examine the molecular recognition properties of biomolecules labelled with inorganic/organometallic reagents by the use of the carbonylmetalloimmunoassay (CMIA) technique. This research will: * Extend the usefulness of capillary electrophoresis in bioanalytical applications, * Explore the chemical selectivity of inorganic/organometallic reagents for biomolecules, * Explore the structural and conformational variances in proteins with inorganic/organometallic reagents, * Examine the changes in chemical reactivity of biomolecules labelled with inorganic/organometallic reagents, and * Develop the carbonylmetalloimmunoassay for the analysis of biomolecule interactions.

本研究将发展毛细管电泳技术 用于研究的合成和分子识别性能， 生物分子特别是那些用无机/有机金属LED标记的生物分子 试剂 研究的具体目标是： * 评估无机/有机金属试剂之间的反应， 肽和蛋白质以及其他生物分子。 * 定义使用CE作为监测微反应器的有用性 生物分子和无机/有机金属试剂的反应， 酶催化反应 * 开发新的合成有机金属试剂和方法， 标记生物分子。 * 测试CE的实用性，以检查的物理有机化学 改性蛋白质 * 检查无机/有机金属标记的 生物分子 * 探索CE在结合分析和非水溶液中的应用， 混合溶剂系统 * 检查标记的生物分子的分子识别特性 与无机/有机金属试剂通过使用 羰基金属免疫分析（CMIA）技术。 这项研究将： * 扩大毛细管电泳在生物分析中的应用 应用程序， * 探索无机/有机金属试剂的化学选择性 对于生物分子， * 探索蛋白质的结构和构象差异， 无机/有机金属试剂， * 检查标记有以下物质的生物分子的化学反应性的变化： 无机/有机金属试剂，和 * 羰基金属免疫分析法在生物分子分析中的应用 交互.