BIOANALYTICAL APPLICATIONS IN CAPILLARY ELECTROPHORESIS

毛细管电泳中的生物分析应用

• 批准号: 6325827
• 负责人: FRANK A GOMEZ
• 金额: $ 7.33万
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6325827
• 关键词: analytical method; binding proteins; bioreactors; capillary electrophoresis; chemical binding; chemical reaction; chemical synthesis; human subject; immunologic assay /test; macromolecule; method development; molecular shape; peptide chemical synthesis; peptides; proteins; reagent /indicator; single cell analysis

The broad goals of this research are to further develop capillary electrophoresis (CE) as a technique to study in-capillary microreactions and to study biomolecular non-covalent interactions. Its focus is on demonstrating principles and on developing useful bioanalytical procedures that can be applied to the analysis of enzyme kinetics and receptor-ligand interactions. Approaches will include in-capillary enzyme-catalyzed microreactions involving both single cell and non- single cell analysis using laser-induced fluorescence (LIF) and UV VIS detection, and affinity capillary electrophoresis (ACE). The research will examine enzymes and small biomolecules that are important in the development of disease and, hence, to the issue of public health. This understanding will strengthen the scientific base underlying the design, preparation, and application of studied. This research effort will provide the technician and graduate student technicians a solid background in chemistry by examining problems at the interface of chemistry, chemistry-related fields, and medicine. The research projects planned for the technicians are aimed at demonstrating the interdisciplinary nature of CE and its importance as a new and exciting instrument in solving broad scientific problems. The specific objectives of the research are to: * Expand the CE-microreactor concept in the analysis of enzyme- catalyzed reactions. * Examine receptor-ligand interactions using affinity CE (ACE). * Study the physical biochemistry of derivatized proteins using CE. This research will: * Extend the usefulness of CE using microscale quantities in bioanalytical applications. * Extend the utility of CE in examining biomolecular non-covalent interactions. * Examine the changes in protein-ligand interactions using covalently modified proteins.

本研究的广泛目标是进一步发展毛细管电泳（CE）作为一种研究毛细管内微反应和研究生物分子非共价相互作用的技术。它的重点是展示原理和开发有用的生物分析程序，可应用于酶动力学和受体-配体相互作用的分析。方法将包括毛细管内酶催化的微反应，涉及使用激光诱导荧光（LIF）和UV维斯检测的单细胞和非单细胞分析，以及亲和毛细管电泳（ACE）。这项研究将检查酶和小生物分子，这些分子在疾病的发展中非常重要，因此对公共卫生问题也很重要。这种理解将加强设计，制备和应用研究的科学基础。这项研究工作将通过检查化学，化学相关领域和医学的界面问题，为技术人员和研究生技术人员提供坚实的化学背景。为技术人员计划的研究项目旨在展示CE的跨学科性质及其作为解决广泛科学问题的新的令人兴奋的工具的重要性。研究的具体目标是：* 在酶催化反应的分析中扩展CE-微反应器概念。* 使用亲和CE（ACE）检查受体-配体相互作用。* 用毛细管电泳研究衍生蛋白质的物理生物化学。这项研究将：* 在生物分析应用中使用微尺度量扩展CE的有用性。* 扩展CE在检测生物分子非共价相互作用中的实用性。* 使用共价修饰的蛋白质检查蛋白质-配体相互作用的变化。