IRIDIUM OXIDE PROTEIN MICROARRAYS

氧化铱蛋白微阵列

• 批准号: 6443581
• 负责人: MICHAEL S WILSON
• 金额: $ 10万
• 依托单位: EIC LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6443581
• 关键词: DNA directed DNA polymerase; biomarker; expression cloning; genotype; iridium; metal oxide; microarray technology; nucleic acid amplification techniques; nucleic acid sequence; technology /technique development; thermostability

DESCRIPTION (provided by applicant): This proposal addresses the need for the development of stable, economical protein microarrays for massive parallel screening of protein interactions. Such arrays will be invaluable in determination of protein expression levels, disease diagnosis, drug discovery, and screening the millions of protein interactions generated by the completion of the human genome. The key innovation of this proposal is the use of an electrochemically grown 3-dimensional hydrous matrix to immobilize proteins and its application in a micro-patterning procedure to fabricate economical microarrays. The use of a 3-dimensional immobilization matrix is expected to reduce the denaturing that can occur in traditional arraying methods where proteins are immobilized on 2-dimensional substrates. In addition, it is anticipated that the larger surface area provided by the porous matrix will allow higher protein loadings, and therefore increased sensitivity, compared to planar substrates. In Phase I, microarrays containing immobilized antibodies will be fabricated, their stability evaluated, and their application in fluorescence based parallel immunoassays and determination of protein expression levels will be demonstrated. In Phase II, higher density protein arrays suitable for integration into automated, high-throughput microfluidic delivery systems and fluorescent analyzers will be developed.

描述（由申请人提供）：本提案涉及以下需求： 开发稳定、经济的蛋白质微阵列， 蛋白质相互作用的筛选。这种阵列将是无价的， 蛋白质表达水平的测定，疾病诊断，药物发现， 并筛选由完成蛋白质合成所产生的数百万种蛋白质相互作用 人类基因组。该提案的主要创新之处在于使用了 电化学生长的三维含水基质， 其在微图案化过程中应用以制造经济 微阵列预期使用三维固定化基质 减少在传统排列方法中可能发生的变性， 蛋白质被固定在二维基质上。此外还 预期由多孔基质提供的较大表面积将 允许更高的蛋白负载，因此与 平面衬底。在第一阶段，含有固定化抗体的微阵列 将制作，其稳定性评价，和他们的应用， 基于荧光的平行免疫测定和蛋白质测定 将展示表达水平。在第二阶段，更高密度的蛋白质 适合集成到自动化、高通量微流体中的阵列 将开发运载系统和荧光分析仪。