Immunoassay Nanocatalysts

免疫测定纳米催化剂

• 批准号: 7612369
• 负责人: MICHAEL S WILSON
• 金额: $ 19.89万
• 依托单位: EIC LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7612369
• 关键词: Agriculture; Alkaline Phosphatase; Amplifiers; Antibodies; Antigens; Bedside Testings; Binding; Biological; Biological Assay; Bioterrorism; Chemicals; Clinical; Color; Cryopreservation; Detection; Diagnostic; Diagnostic tests; Early Diagnosis; Environment; Enzyme-Linked Immunosorbent Assay; Enzymes; Exhibits; Fluorescence; Food; Forensic Medicine; Gold; Heating; Home environment; Horseradish Peroxidase; Hydrogen Peroxide; Immunoassay; Label; Laboratories; Link; Literature; Magnetism; Methods; Modeling; Nanotechnology; Optics; Peroxidases; Phase; Process; Property; Proteomics; Reaction; Reading; Reagent; Refrigeration; Research; Signal Transduction; Soil; Source; System; Temperature; Testing; Time; Water; antibody conjugate; assay development; base; catalyst; cost; drug discovery; enzyme substrate; improved; iridium oxide; mimetics; nanoparticle; novel; particle; programs; public health relevance

DESCRIPTION (provided by applicant): Our aim is to develop a nanotechnology-based biological immunoassay platform that precludes the use of labile enzymes as amplifying labels. Enzyme-labeled antibodies have several well-known limitations. They typically require cold storage or they will lose their activity through denaturation. Furthermore, their activity can be sensitive to the chemical environment. Hence, it would be highly desirable to develop a stable, synthetic system to replace enzymes in this application. Immunoassays are employed in a wide range of biological, agricultural and environmental fields, including clinical diagnostics and point-of-care testing, drug discovery, proteomics, water, food and soil analysis, and forensics (including bioterrorism defense). Robust and cost-effective reagents such as the proposed antibody labels will have a place in all of these arenas. In this Phase I project we will prepare novel antibodies conjugates, evaluate their use in a model immunoassay, and determine their stability at elevated temperatures. The successful completion of these aims will demonstrate that the proposed bioconjugates are potential mimetics and substitutes for enzyme-antibody conjugates used in a multitude of immunoassays. PUBLIC HEALTH RELEVANCE: This project seeks to develop a method for improving the environmental stability of commonly used diagnostic tests. The primary benefit will be that the tests can maintain their high levels of sensitivity without the need for refrigeration. The tests will have applications in a wide range of clinical, biological, agricultural and environmental fields.

描述（由申请人提供）：我们的目标是开发一种基于纳米技术的生物免疫测定平台，防止使用不稳定的酶作为放大标记。酶标记抗体有几个众所周知的局限性。它们通常需要冷藏，否则会因变性而失去活性。此外，它们的活性可能对化学环境敏感。因此，非常需要开发一种稳定的合成系统来替代该应用中的酶。免疫测定广泛应用于生物、农业和环境领域，包括临床诊断和即时检测、药物发现、蛋白质组学、水、食品和土壤分析以及法医学（包括生物恐怖主义防御）。稳健且具有成本效益的试剂（例如拟议的抗体标记）将在所有这些领域占有一席之地。在这个第一阶段项目中，我们将制备新型抗体缀合物，评估它们在模型免疫测定中的用途，并确定它们在高温下的稳定性。这些目标的成功完成将证明所提出的生物缀合物是多种免疫测定中使用的酶-抗体缀合物的潜在模拟物和替代品。 公共健康相关性：该项目旨在开发一种提高常用诊断测试环境稳定性的方法。主要好处是测试可以保持高水平的灵敏度，而无需冷藏。这些测试将广泛应用于临床、生物、农业和环境领域。