Functional Imaging of the Vascular Bed

描述（由申请人提供）：我们的团队最近引入了能够在不需要造影剂的情况下研究血管床的功能特性的工作实时3D成像技术。该技术被称为动态近红外光学断层扫描（DYNOT），采用无害的近红外光源，便携式，适合长时间监测，适用于各种临床环境（包括床边和术中）。在这项研究中，我们计划调查这项技术的实用性，早期检测，微血管病变和神经病变的个人四肢遗传易感合同糖尿病，那些与非胰岛素依赖型糖尿病（NIDDM）和胰岛素依赖型糖尿病（IDDM）和明显的症状，先进的微血管病变。计划的研究旨在检验以下假设：微血管系统的基线时间-频率、自动调节和血管反应性反应的可测量差异将与疾病严重程度相关，如从受试者前臂和小腿收集的时间序列成像数据分析所确定。成像研究将涉及以3 Hz的图像帧速率进行双波长（760和830 nm）断层扫描测量，每次最多20分钟。验证这一假设提供了潜在的特征糖尿病对外周效应机制的最早影响，从而为开发改进的无创监测和治疗方案奠定了基础。

DESCRIPTION (provided by applicant): Our group has recently introduced working real-time 3D-imaging technology capable of investigating the functional properties of the vascular bed without the need of contrast agents. This technology, referred to as Dynamic Near-infrared Optical Tomography (DYNOT), employs harmless near infrared light sources, is portable, suitable for prolonged monitoring, and is adaptable to a wide variety of clinical environments (including bedside and intraoperative). In this study, we plan to investigate the utility of this technology to detect, early-on, microangiopathy and neuropathy in the limbs of individuals genetically predisposed to contract diabetes, those with non-insulin-dependent diabetes (NIDDM) and those with insulin-dependent diabetes (IDDM) and overt symptoms of advanced microangiopathy. Planned studies are designed to test the hypothesis that measurable differences in the baseline time-frequency, auto regulatory and vasoreactivity responses of the microvasculature will correlate with the severity of disease as determined from analysis of time-series imaging data collected from the forearms and calfs of subjects. Imaging studies will involve performing dual wavelength (760 and 830 nm) tomographic measures at an image-framing rate of 3 Hz for up to 20 minutes per session. Validation of this hypothesis offers the potential to characterize the earliest effects of diabetes on the peripheral effector mechanism, thereby setting the stage for development of improved noninvasive monitoring and treatment protocols.