Functional Imaging of Freely Moving Animals

自由活动动物的功能成像

• 批准号: 7274384
• 负责人: RANDALL LOCKE BARBOUR
• 金额: $ 27.95万
• 依托单位: PHOTON MIGRATION TECHNOLOGIES CORP
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7274384
• 关键词: Functional; Imaging; Freely; Moving; Animals

DESCRIPTION (provided by applicant): We propose to develop a dual-mode, optical-EEG sensing system that provides for 4D functional imaging in freely moving animals. The proposed system employs a tethered arrangement that attaches to a two-stage detachable measuring head with one end surgically fixed to the head of a rat. This allows for concurrent collection of whole brain time-series DOT and multilead electrophysiological data while permitting the animal to engage in complex behaviors. Planned are a systematic computational, laboratory and animal studies that will serve to define the limits of performance and fidelity of data quality under realistic conditions. Preliminary data obtained shows that whole head hemodynamic responses gated to behavior dependent hippocampal activation states are spatially distinct and reproducible. The proposed system offers a unique opportunity to explore the dependence of a wide range of complex behaviors on underlying neural and neuro-vascular coupling events and should prove of significant value to the basic neuroscience and pharmaceutical communities.

描述（由申请人提供）：我们建议开发一种双模式光学脑电图传感系统，为自由移动的动物提供 4D 功能成像。所提出的系统采用一种系绳装置，该装置连接到两级可拆卸测量头，其一端通过手术固定到大鼠的头部。这允许同时收集全脑时间序列 DOT 和多导联电生理数据，同时允许动物参与复杂的行为。计划进行系统的计算、实验室和动物研究，以定义现实条件下的性能极限和数据质量的保真度。获得的初步数据表明，与行为依赖的海马激活状态门控的整个头部血流动力学反应在空间上是不同的并且是可重复的。所提出的系统提供了一个独特的机会来探索各种复杂行为对潜在神经和神经血管耦合事件的依赖性，并且应该对基础神经科学和制药界具有重要价值。