Development of a Maskless Array Synthesizer

无掩模阵列合成器的开发

• 批准号: 6526554
• 负责人: ROLAND D GREEN
• 金额: $ 151.55万
• 依托单位: NIMBLEGEN SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6526554
• 关键词: biomedical equipment development; combinatorial chemistry; computer program /software; computer system hardware; genetic polymorphism; genotype; microarray technology; nucleic acid chemical synthesis; oligonucleotides; optics; photochemistry

DESCRIPTION (provided by applicant): NimbleGen will refine its proprietary technology and manufacturing system into the Maskless Array Synthesizer System (MAS Systems). The MAS System will permit owners to rapidly produce inexpensive High Density Custom DNA Arrays (CDAs) in their own facilities to their own specifications. NimbleGen builds its arrays using photo deposition chemistry with its proprietary Maskless UV Light Projector. Texas Instruments Digital Light Processor (DLP) is at the heart of the NimbleGen Projector. The DLP employs a solid-state array of miniaturized aluminum mirrors to pattern up to 780,000 individual pixels of light. Next generation systems will produce up to 1.3 million pixels of light. The computer controlled fabricatoin process requires no human interaction once the glass substrate is placed into NimbleGen's proprietary reaction chamber. Fabrication occurs base-by-base inside the reaction chamber, eliminating the need for the clean rooms common to other array production processes. By using the Maskless UV Projector to control the patterning of UV light on the glass in the reaction chamber, NimbleGen achieves unparalleled precision and control over CDA fabrication. The MAS technology also permits unsurpassed speed - a cycle time of less than four hours - in the production of CDAs, thereby substantially reducing development time and costs. PROPOSED COMMERCIAL APPLICATION: There is a clear need for an inexpensive, rapid, and reliable method of generating and analyzing custom DNA arrays. There are strong indiciations of the proposed project's potential for commercialization, as follows: The market for DNA arrays and DNA array fabrication equipment is projected to be $318 million in 2001, growing to $1.7 billion in 2005, a CAGR of 49%.

描述（由申请人提供）：NimbleGen将完善其专有的 技术和制造系统的无掩模阵列合成器系统 (MAS系统）。MAS系统将允许业主快速生产廉价的 高密度定制DNA阵列（CDA）在自己的设施，以自己的 规范. NimbleGen使用光沉积化学技术构建其阵列， 专利无掩模紫外光投影仪。德州仪器数字灯 处理器（DLP）是NimbleGen投影仪的核心。DLP采用了 固态微型铝镜阵列，图案高达780，000 单个像素的光。下一代系统将产生高达1.3 百万像素的光。计算机控制的制造过程需要 一旦将玻璃基板放入NimbleGen的 专有反应室。制造发生在基地内的基地 反应室，消除了对其他清洁室的需要， 数组生产过程。通过使用无掩模UV投影仪来控制 NimbleGen在反应室的玻璃上形成紫外光图案， 无与伦比的精度和控制能力。MAS技术 还允许无与伦比的速度-循环时间不到四小时-在 生产CDA，从而大大减少开发时间和成本。 拟定商业应用： 显然需要一种廉价、快速和可靠的方法来产生和 分析定制的DNA阵列 有强烈的迹象表明，拟议项目的 商业化的潜力，如下：市场的DNA阵列和DNA阵列 制造设备预计2001年为3.18亿美元，2002年将增长到17亿美元。 2005年，CAGR为49%。