Core--Animal

核心--动物

基本信息

批准号：
6664641
负责人：
PAK H CHAN
金额：
$ 28.95万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-09-27 至 2007-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6664641
关键词：
animal colony biomedical facility brain injury enzyme activity gel electrophoresis gene targeting genetically modified animals immunocytochemistry polymerase chain reaction superoxide dismutase tissue /cell culture

项目摘要

The purpose of this Core is to make available various transgenic and knockout mutants for Projects 1,2, and 3 of the program. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader) will provide his expertise to the other projects in the use of superoxide dismutases, in both transgenic and knockout mutant mice, as well as oxygen radical-related biochemistry and molecular biology. In addition, Dr. Chan will provide his expertise in primary cell culture of neurons, astrocytes and endothelial cells from rodents. This Core will serve as a centralized facility for setting up, for the characterization and the quality control of these primary cell cultures form wild-type animals, as well as SOD1 and SOD2 transgenic and knockout mutant mice. This Core will also provide expertise in the molecular analysis of gene expression (PCR, RT-PCR), gel electrophoresis, enzymatic activity of CuZnSOD and MnSOD and biochemical assays in mice and in primary cell cultures. Finally, this Core will generate and characterize the double transgenic/knockout (SOD1 +/-/ Sod2 -/+) and double knockout (PARP1 -/-/ Sod2 -/+) mice for Projects 1 and 2, respectively. Thus, the Transgenic Animal Core provides a unique vehicle for the projects to achieve the research goals employing various transgenic and knockout animals.

该核心的目的是为项目1,2和3个计划提供各种转基因和敲除突变体。这种转基因动物核心的一个特殊优势是，陈（核心领导者）将在转基因和基因敲除突变小鼠以及氧自由基的生物化学和分子生物学的其他项目中为其他项目提供他的专业知识。此外，Chan博士将在啮齿动物的神经元，星形胶质细胞和内皮细胞的原代细胞培养中提供他的专业知识。该核心将用作设置的集中式设施，用于表征和这些原代细胞培养物的质量控制，形成野生型动物，以及SOD1和SOD2转基因和敲除突变小鼠。该核心还将在小鼠和原发性细胞培养物中的基因表达（PCR，RT-PCR），Cuznsod和Mnsod的酶活性以及生化测定方面提供专业知识。最后，该核心将分别为项目1和2分别生成并表征双转基因/敲除（SOD1+/ - / - /SOD2 - /+）和双敲除（PARP1 - / - /SOD2 - /+）小鼠。因此，转基因动物核心为项目提供了独特的工具，以实现采用各种转基因和淘汰动物的研究目标。