Core--Animal

这个核心的目的是为该计划的项目1、2和3提供各种转基因和基因敲除突变。这个转基因动物核心的一个特别优势是，陈博士(核心领导)将为其他项目提供在转基因和基因敲除突变小鼠中使用超氧化物歧化酶以及与氧自由基相关的生物化学和分子生物学方面的专业知识。此外，陈博士还将提供他在啮齿动物神经元、星形胶质细胞和内皮细胞原代培养方面的专业知识。该中心将作为一个中央设施，用于建立、鉴定和控制野生动物以及SOD1和SOD2转基因和基因敲除突变小鼠的这些原代细胞培养物的质量。该中心还将在基因表达的分子分析(聚合酶链式反应、逆转录聚合酶链式反应)、凝胶电泳法、铜锌超氧化物歧化酶和锰超氧化物歧化酶的酶活性以及小鼠和原代细胞培养中的生化分析方面提供专业知识。最后，该核心将分别为项目1和2产生和表征双转基因/基因敲除(SOD1+/-/Sod2-/+)和双基因敲除(PARP1-/-/Sod2-/+)小鼠。因此，转基因动物核心为项目提供了一个独特的载体，以利用各种转基因和敲除动物来实现研究目标。

The purpose of this Core is to make available various transgenic and knockout mutants for Projects 1,2, and 3 of the program. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader) will provide his expertise to the other projects in the use of superoxide dismutases, in both transgenic and knockout mutant mice, as well as oxygen radical-related biochemistry and molecular biology. In addition, Dr. Chan will provide his expertise in primary cell culture of neurons, astrocytes and endothelial cells from rodents. This Core will serve as a centralized facility for setting up, for the characterization and the quality control of these primary cell cultures form wild-type animals, as well as SOD1 and SOD2 transgenic and knockout mutant mice. This Core will also provide expertise in the molecular analysis of gene expression (PCR, RT-PCR), gel electrophoresis, enzymatic activity of CuZnSOD and MnSOD and biochemical assays in mice and in primary cell cultures. Finally, this Core will generate and characterize the double transgenic/knockout (SOD1 +/-/ Sod2 -/+) and double knockout (PARP1 -/-/ Sod2 -/+) mice for Projects 1 and 2, respectively. Thus, the Transgenic Animal Core provides a unique vehicle for the projects to achieve the research goals employing various transgenic and knockout animals.