Transgenic Animal Core
Transgenic Animal Core
批准号:
7382861
负责人:
PAK H CHAN
金额:
$26.5万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2007
资助国家:
美国
项目状态:
已结题
起止时间:
2007-07-01 至 2012-06-30
关键词:
AnimalsAstrocytesBiochemicalBiochemistryBiological AssayBreedingEndothelial CellsGene ExpressionGenotypeGoalsHeat-Shock Proteins 70Knock-outKnockout MiceMolecular AnalysisMolecular BiologyMusMutant Strains MiceNAD(P)H oxidaseNeuronsPolymerase Chain ReactionPrimary Cell CulturesPurposeQuality ControlReactive Oxygen SpeciesResearchReverse Transcriptase Polymerase Chain ReactionRodentSOD2 geneSuperoxide DismutaseTimeTransgenic AnimalsTransgenic Organismscopper zinc superoxide dismutasegel electrophoresisknockout animalmutantprograms
中文摘要
本核心的目的是为项目1、2、
英文摘要
The purpose of this Core is to make available various transgenic and knockout mutants for Projects 1, 2,
and 3 of the program. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader)
will provide his expertise to the other projects in the use of superoxide dismutases (SOD1, SOD2), in
transgenic and knockout mutant mice, and NAD(P)H oxidase mice (gp91phox and p47phox knockout), as well
as oxygen radical-related biochemistry and molecular biology. In addition, Dr. Chan will provide his expertise
in primary cell culture of neurons (Project 2), astrocytes (Project 3), and endothelial cells (Project 1) from
rodents (transgenic, knockout, and genetically-matched wild-types). This Core will serve as a centralized
facility for set-up, for characterization, and for quality control of these primary cell cultures from wild-type
animals, as well as SOD1 and SOD2 transgenic and knockout mutant mice. In addition, HSP70 transgenic
and PARP-1 knockout mice will be bred and genotyped for Project 3 and Project 2, respectively This Core
will also provide expertise in the molecular analysis of gene expression (PCR, RT-PCR, real-time RT-PCR),
gel electrophoresis, and enzymatic activity of CuZnSOD, MnSOD, NAD(P)H oxidase, and biochemical
assays in mice and in primary cell cultures. Finally, this Core will generate and characterize the double
transgenic/knockout mice (SOD1+/- / gp91phox-/-, SOD2+/- / gp91phox-/-, HSP70+/+ / SOD2-/+) for Projects 1
and 3. Thus, the Transgenic Animal Core provides a unique vehicle for the projects to achieve the research
goals employing various transgenic and knockout animals.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Administrative Core
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批准号:7382863
-
项目类别:
-
资助金额:$13.98万
-
财政年份:2007
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负责人:PAK H CHAN
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依托单位:
Neurovascular Dysfunction, BBB Disruption and Oxidative Stress in Ischemic Brain
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批准号:7382855
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项目类别:
-
资助金额:$51.09万
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财政年份:2007
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负责人:PAK H CHAN
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依托单位:
Core--Transgenic animal
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批准号:6809074
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项目类别:
-
资助金额:$19.41万
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财政年份:2004
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负责人:PAK H CHAN
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依托单位:
Endothelial vascular injury
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批准号:6809066
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项目类别:
-
资助金额:$21.01万
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财政年份:2004
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负责人:PAK H CHAN
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依托单位:
Core--Animal
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批准号:6664641
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项目类别:
-
资助金额:$28.95万
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财政年份:2002
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负责人:PAK H CHAN
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依托单位:
Oxidative stress and metalloproteinases in Bbb injury
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批准号:6664637
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项目类别:
-
资助金额:$28.95万
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财政年份:2002
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负责人:PAK H CHAN
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依托单位:
Core--Transgenic animal
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批准号:6480801
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项目类别:
-
资助金额:$15.58万
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财政年份:2001
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负责人:PAK H CHAN
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依托单位:
Transgenic animal injury paradigms
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批准号:6480800
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项目类别:
-
资助金额:$15.58万
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财政年份:2001
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负责人:PAK H CHAN
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依托单位:
Transgenic animal injury paradigms
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批准号:6396017
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项目类别:
-
资助金额:$18.53万
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财政年份:2000
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负责人:PAK H CHAN
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依托单位:
OXIDATIVE STRESS AND NEURONAL INJURY IN CEREBRAL ISCHEMIA
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批准号:6356582
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项目类别:
-
资助金额:$18.7万
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财政年份:2000
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负责人:PAK H CHAN
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依托单位:
CORE--SCIENTIFIC
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批准号:6356586
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项目类别:
-
资助金额:$18.7万
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财政年份:2000
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负责人:PAK H CHAN
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依托单位:
22ND PRINCETON CONFERENCE ON CEREBROVASCULAR DISEASE
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批准号:6133364
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项目类别:
-
资助金额:$5.8万
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财政年份:2000
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负责人:PAK H CHAN
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依托单位:
Core--Transgenic animal
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批准号:6396018
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项目类别:
-
资助金额:$18.53万
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财政年份:2000
-
负责人:PAK H CHAN
-
依托单位:
Transgenic animal injury paradigms
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批准号:6224479
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项目类别:
-
资助金额:$18.53万
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财政年份:1999
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负责人:PAK H CHAN
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依托单位:
Core--Transgenic animal
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批准号:6224499
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项目类别:
-
资助金额:$18.53万
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财政年份:1999
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负责人:PAK H CHAN
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依托单位:
OXIDATIVE STRESS AND NEURONAL INJURY IN CEREBRAL ISCHEMIA
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批准号:6217892
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项目类别:
-
资助金额:$18.7万
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财政年份:1999
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负责人:PAK H CHAN
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依托单位:
CORE--SCIENTIFIC
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批准号:6217896
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项目类别:
-
资助金额:$18.7万
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财政年份:1999
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负责人:PAK H CHAN
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依托单位:
CORE--SCIENTIFIC
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批准号:6112105
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项目类别:
-
资助金额:$18.7万
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财政年份:1999
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负责人:PAK H CHAN
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依托单位:
Core--Transgenic animal
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批准号:6367032
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项目类别:
-
资助金额:$18.53万
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财政年份:1999
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负责人:PAK H CHAN
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依托单位:
OXIDATIVE STRESS AND NEURONAL INJURY IN CEREBRAL ISCHEMIA
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批准号:6112101
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项目类别:
-
资助金额:$18.7万
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财政年份:1999
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负责人:PAK H CHAN
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依托单位:
国内基金
海外基金
Ascl1介导Wnt/beta-catenin通路在TLE海马硬化中反应性Astrocytes异常增生的作用及调控机制
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批准号:31760279
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项目类别:地区科学基金项目
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资助金额:35.0万元
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批准年份:2017
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负责人:丁银秀
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依托单位: