Core--Transgenic animal

核心--转基因动物

• 批准号: 6809074
• 负责人: PAK H CHAN
• 金额: $ 19.41万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6809074
• 关键词: animal breeding; animal colony; astrocytes; biomedical facility; enzyme activity; free radical oxygen; gel electrophoresis; gene expression; genetically modified animals; laboratory mouse; laboratory rat; molecular biology; neurons; superoxide dismutase; tissue /cell culture; vascular endothelium

The purpose of this core is to make available various transgenic and knockout mutants for Projects by Giffard, Steinberg, and Chan. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader) will provide his expertise to the other projects in the use of superoxide dismutases, in both transgenic and knockout mutant mice, as well as oxygen radical-related biochemistry and molecular biology. In addition, Dr. Chan will provide his expertise in primary cell culture of neurons, astrocytes, and endothelial cells from rodents. This Core will serve as a centralized facility for set-up, characterization and quality control of these primary cell cultures from wild-type animals, as well as SOD1, SOD2, and other transgenic and knockout mutant mice. This Core will also provide expertise in the molecular analysis of gene expression (PCR, RT-PCR), gel electrophoresis, enzymatic activity of CuZnSOD and MnSOD and biochemical assays in mice and in primary cell cultures. In addition, the Core will be used to provide technical assistance in breeding the various transgenic and knockout mice for the various projects of this program. Thus, the Transgenic Animal Core provides a unique vehicle for the projects to achieve the research goals employing various transgenic and knockout animals.

该核心的目的是为Giffard， Steinberg和Chan的项目提供各种转基因和敲除突变体。这个转基因动物核心项目的一个特别优势是，陈博士（核心负责人）将为其他项目提供他的专业知识，包括在转基因和敲除突变小鼠中使用超氧化物歧化酶，以及与氧自由基相关的生物化学和分子生物学。此外，他还将提供他在神经元、星形胶质细胞和啮齿动物内皮细胞的原代细胞培养方面的专业知识。该核心将作为集中设施，用于野生型动物以及SOD1、SOD2和其他转基因和敲除突变小鼠的原代细胞培养物的设置、表征和质量控制。该中心还将提供基因表达的分子分析（PCR, RT-PCR），凝胶电泳，CuZnSOD和MnSOD的酶活性以及小鼠和原代细胞培养的生化分析方面的专业知识。此外，该核心将用于为该计划的各个项目提供育种各种转基因和基因敲除小鼠的技术援助。因此，转基因动物核心为项目提供了一个独特的载体，利用各种转基因和敲除动物来实现研究目标。