CORE--SCIENTIFIC

核心--科学

• 批准号: 6356586
• 负责人: PAK H CHAN
• 金额: $ 18.7万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2002-09-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6356586
• 关键词: animal colony; astrocytes; biomedical facility; brain injury; genetically modified animals; high performance liquid chromatography; immunocytochemistry; in situ hybridization; neurons; polymerase chain reaction; tissue /cell culture

The purpose of the Scientific Core is to make available various transgenic and knockout mutants, primary cell cultures, general molecular biological techniques, biochemical assays, and morphological and histological methods to the four projects of the program. The availability of these services will allow investigators to conduct their studies with consistency in quality control of various methods and assays in a way that would not be possible for individual projects to achieve. One particular strength of this Scientific Core is that all the project leaders are actively participating in the execution of the experiments and provide their own expertise to others during monthly research seminars meetings. For example, Dr. Chan (Core Leader) will provide his expertise in the use of superoxide dismutase transgenic and knockout mutant mice as well as oxygen radicals-related biochemistry and molecular biology. Dr. Sharp will provide his expertise in gene expression (in situ hybridization and immunocytochemistry), DG autoradiography and [14C iodoantipyrine autoradiography for cerebral blood flow. Dr. Noble will offer her expertise in morphological assessments (both light and ultrastructure) of neuronal injury. Dr. Swanson will offer his expertise in HPLC measurements of .OH radicals and H2O2 and quantitative measurements of infarct volume using imaging analysis. In addition, Dr. Sylvia Chen, the coordinator of the Scientific Core, will provide her expertise in molecular analysis of gene expression (PCR, RT-PCR), primary cell culture and various biochemical assays. Thus, this Scientific Core provides an unique vehicle for various projects to achieve their research goals with a unified methodology and expertise that will complement one another.

科学核心的目的是提供各种 转基因和敲除突变体，原代细胞培养物，一般 分子生物学技术，生化测定和形态学 和该计划的四个项目的组织学方法。这 这些服务的可用性将使调查人员能够进行 他们的研究在各种方法的质量控制方面保持一致 并以单个项目不可能的方式进行测定 实现。这个科学核心的一个特殊优势是所有人 项目负责人正在积极参与执行 实验并在月度期间向他人提供自己的专业知识 研究研讨会会议。例如，Chan博士（核心负责人）将 提供他在使用超氧化物歧化酶转基因的专业知识和 敲除突变小鼠以及与氧自由基相关的生物化学 和分子生物学。夏普博士将提供他在基因方面的专业知识 表达（原位杂交和免疫细胞化学），DG 放射自显影和[14c iodoanantipyrine放射自显影术 血流（量。 Noble博士将提供她的形态学专业知识 神经元损伤的评估（光和超微结构）。博士 Swanson将在.OH激进分子的HPLC测量方面提供他的专业知识 H2O2和使用成像对梗塞体积进行定量测量 分析。此外，Sylvia Chen博士 科学核心将提供她在分子分析方面的专业知识 基因表达（PCR，RT-PCR），原代细胞培养和各种 生化测定。因此，这个科学核心提供了独特的 各种项目的工具以实现其研究目标 统一的方法和专业知识将相互补充。