Metabolic engineering of Cupriavidus necator for L-arginine production

Cupriavidus necator 用于生产 L-精氨酸的代谢工程

• 批准号: 1944178
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1944178
• 关键词: Metabolic; engineering; Cupriavidus; necator; arginine

Engineered strains of Corynebacterium glutamicum and Escherichia coli are the workhorses of fermentative amino acid production, a growing industry worth billions. Yet these fermentations rely upon carbon from first-generation biomass feedstocks, which fluctuate in cost and supply.The production of palm oil generates over 30 million tons of carbon-rich waste each year. Together with current methods of fertilisation, these are responsible for at least a third of palm oil greenhouse gas emissions; the equivalent of 60 million tonnes of carbon dioxide.This polluting waste has the potential to be used as a novel feedstock for amino acid fermentation; specifically nitrogen-rich arginine, which is growing in use as a tree fertiliser. Doing so would valorise the waste by recycling its carbon back into plantations, whilst reducing the need for inorganic fertilisers. However, no industrial strain exists for this process.To this end, we aim to engineer a microbial cell factory for the conversion of carbon dioxide to L-arginine. Cupriavidus necator can naturally grow on carbon dioxide and hydrogen, making it an ideal host chassis for this purpose. Using metabolic engineering and synthetic biology strategies, an L-arginine overproducer will be developed and optimised. This will be supported by a complementary range of -omic analyses to provide novel insight into how the biosynthetic pathway is regulated. Once economically viable yields and productivities have been reached, this strain will then be demonstrated at pilot-scale gas fermentation using carbon dioxide as the feedstock.

Corynebacterium glutamicum和Escherichia Coli的工程菌株是发酵氨基酸生产的主力，这是一个价值数十亿美元的行业。然而，这些发酵依赖于第一代生物量原料的碳，这些碳的成本和供应波动。棕榈油的生产每年产生超过3000万吨富含碳的废物。加上当前的受精方法，这些方法至少是棕榈油温室气体排放的三分之一。相当于6000万吨二氧化碳。这种污染废物有可能用作氨基酸发酵的新型原料。特别是富含氮的精氨酸，该精氨酸正在用作树肥。这样做会通过将碳回收回种植园，同时减少对无机肥料的需求，从而使废物恢复废物。但是，此过程不存在任何工业菌株。为了使我们旨在设计微生物细胞工厂，以将二氧化碳转化为L-精氨酸。 Cupriavidus Necator可以自然地在二氧化碳和氢上生长，使其成为为此目的的理想宿主底盘。使用代谢工程和合成生物学策略，将开发和优化L-精氨酸过度生产剂。这将由互补的-omic分析范围支持，以提供有关如何调节生物合成途径的新见解。一旦达到了经济可行的产量和生产力，将在使用二氧化碳作为原料时在试验尺度气体发酵时证明这种菌株。