Core--Image analysis and bioassays

核心--图像分析与生物测定

• 批准号: 6442533
• 负责人: ROBERT C BURGHARDT
• 金额: $ 7.35万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6442533
• 关键词: bioassay; biomedical facility; environmental toxicology; image processing

The Superfund Basic Research Program at Texas A&M University has relied heavily on the facilities and staff support available in the Image Analysis Laboratory due to advances in non-invasive imaging tools using biosensors and biomarkers for defining the function of living cells and tissues. Five different SBRP projects will exploit the analytical imaging technologies within this core to monitor numerous vital functional endpoints within cells exposed to a diverse array of relevant toxicants. Functional endpoints include assessment of intracellular glutathione, pH and Ca2+ homeostasis, intercellular communication, and GFP- tagged protein expression. Additional microscopy support with digital image acquisition and analysis for in situ hybridization and immunocytochemistry will be provided. This Core will provide the interface with the Field Services and Analytical Services Cores receive and/or distribute samples provided by the Field Services of Analytical Services Cores to investigators responsible for performing endocrine disruptor, genotoxicity, or pollutant-sensitive bioassays. Because of its extensive utilization by the different research projects and interactions with other facilities cores, the Image Analysis and Bioassays Core has a strong commitment to advancing the SBRP goals and serves as a forum for scientific interactions between SBRP investigators.

德克萨斯A&M大学的超级基金基础研究项目在很大程度上依赖于图像分析实验室的设施和人员支持，这是由于使用生物传感器和生物标志物来定义活细胞和组织功能的非侵入性成像工具的进步。五个不同的SBRP项目将利用该核心中的分析成像技术来监测暴露于各种相关毒物的细胞内的许多重要功能终点。功能终点包括细胞内谷胱甘肽、pH和Ca 2+稳态、细胞间通讯和GFP标记的蛋白质表达的评估。将提供额外的显微镜支持，包括原位杂交和免疫细胞化学的数字图像采集和分析。该中心将提供与现场服务和分析服务中心的接口，接收和/或分发分析服务中心现场服务提供的样本给负责进行内分泌干扰物、遗传毒性或污染物敏感性生物测定的研究者。由于其被不同的研究项目广泛利用，并与其他设施的核心相互作用，图像分析和生物测定核心有一个坚定的承诺，以推进SBRP的目标，并作为SBRP研究人员之间的科学互动的论坛。