THEORY OF MULTI PHOTON EXCITATION

多光子激发理论

• 批准号: 6444724
• 负责人: PATRIK R CALLIS
• 金额: $ 29.31万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444724
• 关键词: biological products; biomedical resource; informatics; spectrometry; technology /technique

Theoretical calculations of electronic transitions in organic molecules for different modes of excitation allow one to predict the cross-section and fundamental anisotropy for multi-photon excitation. The fundamental anisotropy of NADH is near 0.54 for two-photon excitation from 690-740 nm, which is 46% higher than the value of 0.37 observed for one-photon excitation. This observation of a higher anisotropy with two-photon excitation was consistent with INDO/SDCI calculation of the one- and two-photon transition. NADH displays a low cross-section for two photon excitation, which suggest that fluorescence from NADH will be negligible for two-photon fluorescence microscopy and will not significantly interfere with observations of TPIF of other extrinsic probes used to label cells.

有机分子电子跃迁的理论计算 不同激发模式的分子允许人们预测 截面和多光子激发的基本各向异性。 对于双光子，NADH的基本各向异性接近0.54 从690-740 nm激发，这比0.37的值高46%， 观察到单光子激发。 这是一个更高的观察 双光子激发的各向异性与INDO/SDCI一致 单光子和双光子跃迁的计算。 NADH显示a 双光子激发的低截面，这表明， 对于双光子荧光，来自NADH的荧光将是可忽略的 显微镜检查，并不会显着干扰观察 用于标记细胞的其他外源性探针的TPIF。