MECHANISM OF TRYPTOPHAN FLUORESCENCE QUENCHING
MECHANISM OF TRYPTOPHAN FLUORESCENCE QUENCHING
批准号:
6444705
负责人:
BOGUMIL ZELENT
金额:
$29.31万
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-04-01 至 2002-03-31
中文摘要
我们检查了时间分辨和稳态荧光
英文摘要
We examined the time-resolved and steady-state fluorescence
quenching of N-acetyl-L-tryptophanamide. (NATA) by acrylamide and
iodide, over a range of viscosities in propylene glycol. The
quenching of NATA by acrylamide and iodide results in heterogeneity of
the intensity decay which increases with the quencher concentration.
We attribute the complex decay of NATA to transient effects in
diffusion and the nature of the fluorophore-quencher interaction.
Consideration of both the frequency-domain and steady-state data
demonstrate that the quenching rate depends exponentially on the
fluorophore-quencher distance, indicating the validity of a model
which include a quenching constant which depend exponentially on
distance. Such data on the distance dependence of quenching is
important for understanding quenching of native proteins.
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MECHANISM OF TRYPTOPHAN FLUORESCENCE QUENCHING
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批准号:7182004
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项目类别:
-
资助金额:$1.03万
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财政年份:2005
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负责人:BOGUMIL ZELENT
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依托单位:
MECHANISM OF TRYPTOPHAN FLUORESCENCE QUENCHING
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批准号:6978331
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项目类别:
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资助金额:$2.2万
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财政年份:2004
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负责人:BOGUMIL ZELENT
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依托单位:
MECHANISM OF TRYPTOPHAN FLUORESCENCE QUENCHING
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批准号:6315371
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项目类别:
-
资助金额:$2.83万
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财政年份:2000
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负责人:BOGUMIL ZELENT
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依托单位:
MECHANISM OF TRYPTOPHAN FLUORESCENCE QUENCHING
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批准号:6319881
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项目类别:
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资助金额:$0.85万
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财政年份:1999
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负责人:BOGUMIL ZELENT
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依托单位:--
MECHANISM OF TRYPTOPHAN FLUORESCENCE QUENCHING
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批准号:6282913
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项目类别:
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资助金额:$3.48万
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财政年份:1998
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负责人:BOGUMIL ZELENT
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依托单位:
海外基金