MECHANISM OF TRYPTOPHAN FLUORESCENCE QUENCHING

色氨酸荧光猝灭机理

• 批准号: 6444705
• 负责人: BOGUMIL ZELENT
• 金额: $ 29.31万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444705
• 关键词: biological products; biomedical resource; informatics; spectrometry

We examined the time-resolved and steady-state fluorescence quenching of N-acetyl-L-tryptophanamide. (NATA) by acrylamide and iodide, over a range of viscosities in propylene glycol. The quenching of NATA by acrylamide and iodide results in heterogeneity of the intensity decay which increases with the quencher concentration. We attribute the complex decay of NATA to transient effects in diffusion and the nature of the fluorophore-quencher interaction. Consideration of both the frequency-domain and steady-state data demonstrate that the quenching rate depends exponentially on the fluorophore-quencher distance, indicating the validity of a model which include a quenching constant which depend exponentially on distance. Such data on the distance dependence of quenching is important for understanding quenching of native proteins.

我们检查了时间分辨和稳态荧光 N-乙酰基-L-色氨酸酰胺的猝灭。 (NATA) 由丙烯酰胺和 碘化物，在丙二醇中具有一定的粘度。 这 丙烯酰胺和碘化物猝灭 NATA 导致不均匀性 强度衰减随着猝灭剂浓度的增加而增加。 我们将 NATA 的复杂衰减归因于 扩散和荧光团-猝灭剂相互作用的性质。 考虑频域和稳态数据 证明淬火速率呈指数关系 荧光团-猝灭剂距离，表明模型的有效性 其中包括一个淬灭常数，该常数呈指数关系 距离。 这些关于淬火距离依赖性的数据是 对于理解天然蛋白质的猝灭很重要。