Engineering long-acting oral enzymes for animal nutrition through site-specific anchorage

通过特定位点锚定设计用于动物营养的长效口服酶

• 批准号: 1947331
• 金额: --
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1947331
• 关键词: Engineering; long; acting; oral; enzymes

Phosphorus is a key mineral nutrient for animal growth. Phytic acid is the main storage form of phosphorus in plant-based feeds. Phosphorus in phytic acid, however, is not available to non-ruminant animals such as poultry and swine. One solution to the problem of low available phosphorus in animal feed is supplementing feed with phytase. Phytase is an enzyme that releases phosphate from phytic acid. The aim of this project is to develop a means of anchoring phytase in the gizzard, its main site of action in the gastrointestinal tract. We hypothesize that improved phytase retention in the gizzard will improve the enzyme's efficacy. The main work of this project will be to select a binding protein specific to the gizzard using a phage display platform adapted to the gastric environment. The selected binding protein can then be genetically fused to phytase and the impact on the enzyme's efficacy assessed. It is intended that the platform developed may facilitate future targeting applications in the gastrointestinal tract. IBB, ENWW

磷是动物生长的关键矿物质营养素。植酸是植物性饲料中磷的主要贮存形式。然而，植酸中的磷不能被非反刍动物如家禽和猪利用。解决饲料中有效磷含量低的方法之一是在饲料中添加植酸酶。植酸酶是一种从植酸中释放磷酸盐的酶。该项目的目的是开发一种将植酸酶锚定在胃肠道主要作用部位--砂囊中的方法。我们假设，改善植酸酶保留在砂囊将提高酶的效力。本项目的主要工作是利用适合胃内环境的噬菌体展示平台，筛选出一种特异性结合蛋白。然后可以将所选择的结合蛋白与植酸酶基因融合，并评估对酶功效的影响。预期开发的平台可促进未来在胃肠道中的靶向应用。伊布，ENWW