ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA

描述（申请中的逐字记录）：此申请的目的 目的是了解 CHD 蛋白在发育决定中的作用 拟南芥中的身份。 CHD 蛋白被认为是 染色质重塑因子存在于许多不同的生物体中，包括 酵母对人。在拟南芥中，pkl 突变在 赤霉素信号转导途径及其在发育转变中的作用 形成胚芽至幼苗。 PKL 已被克隆并编码为 CHD 家庭成员。 我们有兴趣了解这种 CHD 蛋白在调节中的作用 发育特性和 GA 信号转导的研究。识别可能的 PKL 的调节机制、PKL 转录物和蛋白的表达 将被表征。将鉴定直接受以下因素调控的基因 PKL 因此可能参与发育身份的调节 和GA信号转导。与 PKL 相互作用的蛋白质将被鉴定 通过双杂交分析和亲和层析试图鉴定 可能调节 PKL 活性的潜在辅助因子。其他因素 参与发育特性、赤霉素信号的调节 转导和/或染色质重塑可以基于遗传来识别 屏幕。通过实现这些实验目标，希望能有更大的成果 将了解 CHD 蛋白在调节中的作用 植物乃至所有真核生物的发育。

DESCRIPTION (Verbatim from the application): The objective of this application is to understand the role of a CHD protein in determination of developmental identity in Arabidopsis thaliana. CHD proteins have been implicated as chromatin remodeling factors are found in many diverse organisms ranging from yeast to man. In Arabidopsis thaliana, the pkl mutation is defective in the gibberellin signal transduction pathway and in the developmental transition form embryo to seedling. PKL has been cloned and codes for a CHD family member. We are interested in understanding the role of this CHD protein in regulation of developmental identity and GA signal transduction. To identify possible mechanisms by which PKL is regulated, expression of PKL transcript and protein will be characterized. Genes will be identified that are directly regulated by PKL and therefore potentially involved in regulation of developmental identity and GA signal transduction. Proteins that interact with PKL will be identified by two-hybrid analysis and affinity chromatography in an attempt to identify potential co-factors that may regulate PKL activity. Additional factors that are involved in regulation of developmental identity, gibberellin signal transduction, and/or chromatin remodeling may be identified based on a genetic screen. By carrying out these experimental aims, it is hoped that a greater understanding will be achieved of the role of CHD proteins in regulation of development in plants and perhaps all eukaryotes.