ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA
ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA
批准号:
6520066
负责人:
JOSEPH P OGAS
金额:
$21.11万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-04-01 至 2005-03-31
中文摘要
描述(申请中的逐字记录):此申请的目的
目的是了解 CHD 蛋白在发育决定中的作用
拟南芥中的身份。 CHD 蛋白被认为是
染色质重塑因子存在于许多不同的生物体中,包括
酵母对人。在拟南芥中,pkl 突变在
赤霉素信号转导途径及其在发育转变中的作用
形成胚芽至幼苗。 PKL 已被克隆并编码为 CHD 家庭成员。
我们有兴趣了解这种 CHD 蛋白在调节中的作用
发育特性和 GA 信号转导的研究。识别可能的
PKL 的调节机制、PKL 转录物和蛋白的表达
将被表征。将鉴定直接受以下因素调控的基因
PKL 因此可能参与发育身份的调节
和GA信号转导。与 PKL 相互作用的蛋白质将被鉴定
通过双杂交分析和亲和层析试图鉴定
可能调节 PKL 活性的潜在辅助因子。其他因素
参与发育特性、赤霉素信号的调节
转导和/或染色质重塑可以基于遗传来识别
屏幕。通过实现这些实验目标,希望能有更大的成果
将了解 CHD 蛋白在调节中的作用
植物乃至所有真核生物的发育。
英文摘要
DESCRIPTION (Verbatim from the application): The objective of this application
is to understand the role of a CHD protein in determination of developmental
identity in Arabidopsis thaliana. CHD proteins have been implicated as
chromatin remodeling factors are found in many diverse organisms ranging from
yeast to man. In Arabidopsis thaliana, the pkl mutation is defective in the
gibberellin signal transduction pathway and in the developmental transition
form embryo to seedling. PKL has been cloned and codes for a CHD family member.
We are interested in understanding the role of this CHD protein in regulation
of developmental identity and GA signal transduction. To identify possible
mechanisms by which PKL is regulated, expression of PKL transcript and protein
will be characterized. Genes will be identified that are directly regulated by
PKL and therefore potentially involved in regulation of developmental identity
and GA signal transduction. Proteins that interact with PKL will be identified
by two-hybrid analysis and affinity chromatography in an attempt to identify
potential co-factors that may regulate PKL activity. Additional factors that
are involved in regulation of developmental identity, gibberellin signal
transduction, and/or chromatin remodeling may be identified based on a genetic
screen. By carrying out these experimental aims, it is hoped that a greater
understanding will be achieved of the role of CHD proteins in regulation of
development in plants and perhaps all eukaryotes.
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会议论文
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批准号:8624289
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项目类别:
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资助金额:$19.33万
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财政年份:2013
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负责人:JOSEPH P OGAS
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依托单位:
ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA
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批准号:6127992
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项目类别:
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资助金额:$23.07万
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财政年份:2000
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负责人:JOSEPH P OGAS
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依托单位:
ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA
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批准号:6732670
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项目类别:
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资助金额:$26.69万
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财政年份:2000
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负责人:JOSEPH P OGAS
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依托单位:
ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA
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批准号:6386573
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项目类别:
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资助金额:$24.77万
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财政年份:2000
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负责人:JOSEPH P OGAS
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依托单位:
ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA
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批准号:6636331
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项目类别:
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资助金额:$26.54万
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负责人:JOSEPH P OGAS
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依托单位:
ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA
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批准号:6599917
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项目类别:
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资助金额:$3.97万
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财政年份:2000
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负责人:JOSEPH P OGAS
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依托单位:
Cancer Research Career Enhancement and Related Activities
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批准号:10658857
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项目类别:
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资助金额:$3.27万
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财政年份:1998
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负责人:JOSEPH P OGAS
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依托单位:
Cancer Research Career Enhancement and Related Activities
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批准号:10223199
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项目类别:
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资助金额:$3.27万
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财政年份:1998
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负责人:JOSEPH P OGAS
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依托单位:
Cancer Research Career Enhancement and Related Activities
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批准号:10434751
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项目类别:
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资助金额:$3.27万
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财政年份:1998
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依托单位:
Cancer Research Career Enhancement and Related Activities
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批准号:10024907
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项目类别:
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资助金额:$3.27万
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财政年份:--
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负责人:JOSEPH P OGAS
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依托单位:
海外基金