ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA

描述（来自申请的逐字）：本申请的目的 是了解CHD蛋白在决定发育中的作用， 拟南芥中的同一性。CHD蛋白被认为是 染色质重塑因子存在于许多不同的生物体中， 在拟南芥中，pkl突变在酵母中是有缺陷的。 赤霉素信号转导途径与发育转型 从胚到幼苗。PKL已被克隆并编码CHD家族成员。 我们有兴趣了解这种CHD蛋白在调节 的发育特性和GA信号转导。查明可能 PKL的调控机制，PKL转录物和蛋白质的表达 将被定性。基因将被鉴定为直接受 PKL，因此可能参与发育身份的调节 和GA信号转导。将鉴定与PKL相互作用的蛋白质 通过双杂交分析和亲和层析， 可能调节PKL活性的潜在辅助因子。的附加因素 参与调节发育特性、赤霉素信号 转导和/或染色质重塑可以基于遗传修饰来鉴定。 屏幕通过实施这些实验目标，人们希望， 了解CHD蛋白质在调节 在植物甚至所有真核生物中的发育。

DESCRIPTION (Verbatim from the application): The objective of this application is to understand the role of a CHD protein in determination of developmental identity in Arabidopsis thaliana. CHD proteins have been implicated as chromatin remodeling factors are found in many diverse organisms ranging from yeast to man. In Arabidopsis thaliana, the pkl mutation is defective in the gibberellin signal transduction pathway and in the developmental transition form embryo to seedling. PKL has been cloned and codes for a CHD family member. We are interested in understanding the role of this CHD protein in regulation of developmental identity and GA signal transduction. To identify possible mechanisms by which PKL is regulated, expression of PKL transcript and protein will be characterized. Genes will be identified that are directly regulated by PKL and therefore potentially involved in regulation of developmental identity and GA signal transduction. Proteins that interact with PKL will be identified by two-hybrid analysis and affinity chromatography in an attempt to identify potential co-factors that may regulate PKL activity. Additional factors that are involved in regulation of developmental identity, gibberellin signal transduction, and/or chromatin remodeling may be identified based on a genetic screen. By carrying out these experimental aims, it is hoped that a greater understanding will be achieved of the role of CHD proteins in regulation of development in plants and perhaps all eukaryotes.