ANALYSIS OF ROLE OF CHD PROTEINS IN ARABIDOPSIS THALIANA

拟南芥CHD蛋白的作用分析

• 批准号: 6636331
• 负责人: JOSEPH P OGAS
• 金额: $ 26.54万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6636331
• 关键词: Arabidopsis; DNA binding protein; affinity chromatography; biological signal transduction; phytohormones; plant genetics; plant growth /development; protein structure function; yeast two hybrid system

DESCRIPTION (Verbatim from the application): The objective of this application is to understand the role of a CHD protein in determination of developmental identity in Arabidopsis thaliana. CHD proteins have been implicated as chromatin remodeling factors are found in many diverse organisms ranging from yeast to man. In Arabidopsis thaliana, the pkl mutation is defective in the gibberellin signal transduction pathway and in the developmental transition form embryo to seedling. PKL has been cloned and codes for a CHD family member. We are interested in understanding the role of this CHD protein in regulation of developmental identity and GA signal transduction. To identify possible mechanisms by which PKL is regulated, expression of PKL transcript and protein will be characterized. Genes will be identified that are directly regulated by PKL and therefore potentially involved in regulation of developmental identity and GA signal transduction. Proteins that interact with PKL will be identified by two-hybrid analysis and affinity chromatography in an attempt to identify potential co-factors that may regulate PKL activity. Additional factors that are involved in regulation of developmental identity, gibberellin signal transduction, and/or chromatin remodeling may be identified based on a genetic screen. By carrying out these experimental aims, it is hoped that a greater understanding will be achieved of the role of CHD proteins in regulation of development in plants and perhaps all eukaryotes.

描述（来自应用程序）：此应用程序的目的