Candida albicans oral biofilm

白色念珠菌口腔生物膜

• 批准号: 6524140
• 负责人: Welda LaJean CHAFFIN
• 金额: $ 33.3万
• 依托单位: TEXAS TECH UNIVERSITY HEALTH SCIS CENTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6524140
• 关键词: Candida albicans; bacteria infection mechanism; clinical research; computer data analysis; fungal genetics; gene environment interaction; gene expression; human tissue; microarray technology; molecular film; mycosis; nucleic acid probes; opportunistic infections; oral bacteria; polymerase chain reaction; regulatory gene; scanning electron microscopy

DESCRIPTION (provided by applicant): Candida albicans is a commensal that colonizes skin and mucosal surfaces including the oral cavity. The organism is also an agent of opportunistic disease of these surfaces as well as internal disseminated disease. Oral candidiasis is associated with derangements of the oral flora related with the acquisition of microbes by neonates and anti-bacterial therapy, oral prostheses, and host factors such as diabetes mellitus and HIV infection. Oral manifestations include pseudomembraneous candidiasis (thrush) and denture stomatitis. Oropharyngeal infection is virtually an inescapable consequence of AIDS (96 percent patients) and frequently reoccurs. Denture stomatitis may affect 50 percent of complete denture wearers. The organism forms biofilms on mucosa, teeth and oral devices such as dentures, generally in association with oral bacteria. Compared to planktonic cells, organisms in biofilms have characteristics such as reduced susceptibility to antifungal drugs and the presence of an extracellular matrix. This study will test the hypothesis that unique characteristics associated with C. albicans biofilms are the result of altered gene expression in general cellular metabolism as well as bioflim specific gene expression. A model of saliva-coated denture acrylic established in this laboratory will be used. About 230 alterations in general cellular metabolism have been identified in biofilm compared to planktonic cells by exploiting the high homology between Saccharomyces cerevisiae and C. albicans and the commercial availability of gene arrays for S. cerevisiae. In Aim 1 this approach will be applied to examine expression temporally during biofilm formation and to other conditions of biofilm development using C. albicans DNA chips. In Aim 2, expression in in vitro biodiverse models will also be examined to identify genes inherently associated with biofilms as differentiated from those influenced by the biofilm environment. Expression of selected genes from the inherent biofilm expression class will be determined in vivo in organisms recovered from human saliva. Aim 3 will examine the role of biofilm-regulated genes such as TUP1and EFG1 using genetically modified strains.

描述（由申请人提供）：白色念珠菌是一种真菌， 定植于皮肤和粘膜表面，包括口腔。所述生物体是 也是这些表面以及内部的机会性疾病的病原体 传播性疾病口腔念珠菌病与口腔粘膜的紊乱有关， 与新生儿获得微生物有关的口腔植物群， 抗菌治疗、口腔修复和宿主因素如糖尿病 糖尿病和HIV感染。口腔表现包括假膜性 念珠菌病（鹅口疮）和义齿性口炎。口咽感染是 几乎是艾滋病不可避免的后果（96%的患者）， 经常复发。皮炎可能会影响50%的完整的 戴假牙的人该微生物在粘膜、牙齿和口腔装置上形成生物膜 例如假牙，通常与口腔细菌有关。相比 在生物膜中的浮游细胞、生物体具有诸如减少的 对抗真菌药物的敏感性和细胞外基质的存在。 这项研究将测试的假设，独特的特点与 C.白念珠菌生物膜通常是基因表达改变的结果 细胞代谢以及生物膜特异性基因表达。的典范 将使用本实验室建立的唾液涂覆的义齿丙烯酸。 在一般细胞代谢中已经鉴定了大约230种改变， 通过利用生物膜与细胞之间的高度同源性， 酿酒酵母（Saccharomyces cerevisiae）和C.白色念珠菌和商业可用性 S.啤酒。在目标1中，这种方法将适用于 在生物膜形成期间和其他条件下暂时检查表达 的生物膜发育，使用C.白色念珠菌DNA芯片在目标2中， 体外生物多样性模型也将被检查，以确定基因固有的 与受生物膜影响的生物膜相区别 环境从固有生物膜表达中选择的基因的表达 类将在从人唾液中回收的生物体中体内测定。目的 3将研究生物膜调节基因的作用，如TUP1和EFG1，使用 转基因菌株