COMBINATORIAL DETERMINATION OF ISOTOPE EFFECTS

描述：(首席调查员摘要)基于我们的后续 发展强大而快速的反应研究方法学 机制方面，我们提出了研究机制的一系列重要内容 有机、有机金属和生物有机反应。这包括Coarctate 铜酸盐的反应，共轭加成，烯烃歧化，加成 烯烃和亚膦到烯烃的酶促脱羧化反应 氧化角鲨烯的酶促环化反应，以及许多其他反应。这个 我们方法论的独特能力应该会显著提升 对这些反应的机械理解。我们还计划延长我们的 方法论通过开发更强大的反应机理和 结构和分子间相互作用的同位素效应探针。这个 这项工作与健康的相关性源于它对 在医学上重要的物质的合成中的重要反应和 在生物合成途径中很重要的反应。

DESCRIPTION: (Principal Investigator's Abstract) Based on our continued development of powerful and rapid methodology for the study of reaction mechanisms, we propose to study the mechanisms for a series of important organic, organometallic, and bioorganic reactions. This includes coarctate reactions, conjugate additions to cuprates, olefin metathesis, additions of ketenes and phosphinidenes to alkenes, the enzymatic decarboxylation of orotate, the enzymatic cyclization of squalene oxide, and many others. The unique capabilities of our methodologies should significantly advance mechanistic understanding of these reactions. We also plan to extend our methodology by developing more robust probes of reaction mechanisms and isotope-effect probes of structure and intermolecular interactions. The health-relatedness of this work derives from its impact on the understanding of reactions important in the synthesis of medicinally important substances and reactions important in biosynthetic pathways.