The development of an organ on a chip platform.

芯片平台上器官的开发。

• 批准号: 2008256
• 金额: --
• 依托单位: Brunel University London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2008256
• 关键词: development; organ; chip; platform.

Aim:To design and develop a low cost Organ On a Chip platform that can be 3D printed using an Ultimaker 2plus. This platform must be powered using batteries as it considers the use in 3rd world countries where power cuts may occur often.Key research questions: How to seal the enclosure to ensure a 70 per cent CO2 environment?How to utilize the space within the enclosure to ensure all components fit, due to the size constraint as the aim is to use an Ultimaker 2+ 3D printing machine?Investigate various methods/materials to ensure the cells can be analysed under a microscope.Which coding platform will be used to control the sensors and pump (Arduino or Raspberry pie)?Will a GUI (Graphical User Interface) be needed to display the ambient environment within the enclosure, or to control the pump?Which power system will be used to allow the platform to last for more than 30 consecutive days?Brief methodology:Initially, design requirements and specifications must be outlined in order to construct quantitative statements to guide the design process. Subsequently, as stated in the abstract, the current Organ on a Chip platforms will be evaluated against these design specifications to analyse the effectiveness and limitations of each system. Conceptual designs will be assessed by using a weighted decision matrix to select an effective design to fabricate. Prior to fabrication, Computational Fluid Dynamics (CFD) can be used to visualise and investigate the effects of the fluid flow (microfluidics flow), and the pressure applied on the design. The material selection of the heating pad, enclosure and the chip itself will be conducted in conjunction with the design embodiment. The material considered must be able to withstand the heat pad, affordable and transparent. Having achieved the optimal design with the most effective material, the enclosure can be fabricated using the Ultimaker 2+ 3D printing machine, as this machine is affordable and available at the Partnership University in Uganda. The prototype will be tested using the correct working conditions of cell culture in order to appraise the effectiveness of the Organ on a Chip platform against the design specifications and objectives.In conjunction with the enclosure design other factors of the platform must be investigated, these include; Which sensors will be used (Temperature, humidity, PH levels)?The pumping mechanism.The hot plate that the chip will sit on.Where the waste will be kept for further testing.The chip design and the fabrication method. This is crucial due to the cell visualization limitations that 3D printing has. The cell culture must be enclosed in a clear enclosure so it can be analsysed under a microscope.Once a full working platform has been achieved, further investigations regarding the cell culture scaffolding will be conducted. Metrigel and Collagen scaffolds could be considered to grow breast cancer cells.

目的：设计和开发一种低成本的器官芯片平台，可以使用Ultimaker 2plus进行3D打印。该平台必须使用电池供电，因为它考虑到在经常发生停电的第三世界国家使用。关键研究问题：如何密封外壳以确保70%的二氧化碳环境？由于尺寸限制，如何利用外壳内的空间来确保所有组件都适合，因为目标是使用Ultimaker 2 + 3D打印机？研究各种方法/材料，以确保细胞可以在显微镜下分析。哪个编码平台将用于控制传感器和泵（Arduino或Raspberry pie）？是否需要GUI（图形用户界面）来显示外壳内的周围环境或控制泵？将采用哪种动力系统，让平台连续续航超过30天？简要方法：首先，必须概述设计要求和规范，以便构建定量陈述来指导设计过程。随后，如摘要所述，将根据这些设计规范对当前的芯片器官平台进行评估，以分析每个系统的有效性和局限性。概念设计将通过使用加权决策矩阵进行评估，以选择有效的设计进行制造。在制造之前，计算流体动力学（CFD）可用于可视化和研究流体流动（微流体流动）以及施加在设计上的压力的影响。加热垫、外壳和芯片本身的材料选择将结合设计实施例进行。考虑的材料必须能够承受热垫，负担得起的和透明的。在使用最有效的材料实现最佳设计后，可以使用Ultimaker 2 + 3D打印机制造外壳，因为这台机器价格实惠，可在乌干达的伙伴关系大学使用。原型将使用正确的细胞培养工作条件进行测试，以评估器官芯片平台的有效性是否符合设计规范和目标。结合外壳设计，必须研究平台的其他因素，包括：将使用哪些传感器（温度，湿度，PH值）？泵送机制。芯片将坐在上面的热板。废物将被保存以供进一步测试。芯片设计和制造方法。由于3D打印的细胞可视化限制，这一点至关重要。细胞培养物必须封闭在一个透明的外壳中，以便在显微镜下进行分析。一旦达到完整的工作平台，将进行关于细胞培养支架的进一步研究。Metrigel和胶原蛋白支架可以被认为是生长乳腺癌细胞。