Genetics Of Obesity And Energy Metabolism

该项目的主要目的是确定人类肥胖的遗传和/或分子原因。我们使用基因测序技术来鉴定肥胖靶基因的多态性。我们使用人类神经生物学微阵列来研究肥胖和瘦弱成年捐赠者死后脑样本中的差异基因表达。在过去的一年里，我们已经：a)排除了先前由AFLP鉴定的染色体11、15、16和X上的4个小区域的作用，这些区域可能包含与肥胖相关的多态性；b)研究胰岛素基因（INS） VNTR区域的snp；c)开始鉴定脂肪酸合成酶基因（FAS）的snp；D)发起了与其他机构的合作，建立了一个专门储存肥胖捐赠者大脑样本的脑库；E)完成3例肥胖和3例瘦弱供体下丘脑基因表达实验；f)启动了一项探索性研究，利用蛋白质组学鉴定太平洋人和高加索人脑脊液中分泌的差异蛋白

The main aim of this projects is to identify the genetic and/or molecular cause(s) of obesity in humans. We use gene sequencing techniques to identify polymorphisms in obesity target genes. We use a human neurobiology microarray to study the differential gene expression in post-mortem brain samples from obese and lean adult donors. In the past year we have: a) excluded the role of 4 small regions on chromosome 11, 15, 16 and X previoulsy identified by AFLP as potentially harboring polymorphisms linked to obesity; b) studied SNPs in the VNTR region of the insulin gene (INS); c) initiated the identification of SNPs in the fatty acid synthase gene (FAS); d)initiated a collaborative effort with other institutions to create a brain bank specialized in brain samples from obese donors; e) completed gene expression exeriments in the hypothalami of 3 obese and 3 lean donors; f) initiated an exploratory study to use proteomics for the identification of proteins differentially secreted in the CSF of Pimas and Caucasians