Association Fiber Induced Plasticity In Olfactory Cortex

嗅觉皮层中关联纤维诱导的可塑性

• 批准号: 6487638
• 负责人: HEATHER L GILMORE
• 金额: $ 2.45万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6487638
• 关键词: afferent nerve; electrical measurement; electrodes; electrophysiology; evoked potentials; histology; intracellular; laboratory rat; membrane potentials; neural information processing; neural plasticity; neurons; odors; olfactory lobe; olfactory stimulus; predoctoral investigator; pyramidal cells

DESCRIPTION (provided by applicant): The goal of the proposed research is to increase understanding of olfactory processing and the role played in olfactory plasticity by the olfactory cortex association fiber system. High-frequency stimulation of olfactory cortex association fibers has previously been shown to induce a novel form of potentiation in evoked field potentials. The proposed research will investigate the events underlying this potentiation using intracellular recordings from neurons in the olfactory cortex of intact animals, which permits the study of intracellular effects while preserving the network properties of the cortex. Preliminary data indicate that the primary intracellular effect produced by high-frequency association fiber stimulation, repeated daily for 4 to 6 days, is a pronounced hyperpolarization of the membrane potential of olfactory cortex pyramidal cells. The proposed research will further characterize this intracellular response and explore its functional significance. Specific aims of the proposed research are to: 1) compare the intracellular effects of high-frequency association fiber stimulation in naive vs. previously potentiated animals to determine whether prior potentiation is necessary to produce this effect; 2) determine whether the intracellular hyperpolarization is accomplished by enhanced inhibition or reduced synaptic drive (disfaciliation); and 3) examine the effect of the potentiation on the response of olfactory cortex pyramidal cells to stimulation of the afferent pathway to the cortex (lateral olfactory tract), tested with both electrical stimulation and naturally occurring odors. This research will provide an important contribution to the understanding of olfactory processing, thus aiding in the understanding and eventual treatment of olfactory disorders.

描述（由申请人提供）：拟议研究的目标是 增加对嗅觉处理及其在嗅觉中所扮演的角色的了解 嗅觉皮层关联纤维系统的可塑性。高频 先前已证明刺激嗅觉皮层关联纤维 在诱发场电位中诱导一种新的增强形式。拟议的 研究将调查这种增强作用背后的事件 来自完整动物嗅觉皮层神经元的细胞内记录， 允许在保留网络的同时研究细胞内效应 皮质的特性。初步数据表明，主要 高频关联纤维刺激产生的细胞内效应， 每天重复 4 到 6 天，是明显的超极化 嗅皮层锥体细胞的膜电位。拟议的研究 将进一步表征这种细胞内反应并探索其功能 意义。拟议研究的具体目标是：1）比较 高频关联纤维刺激的细胞内效应 与先前增强的动物进行比较，以确定先前的增强是否是 产生这种效果所必需的； 2) 判断细胞内是否 超极化是通过增强抑制或减少突触来实现的 驾驶（妨碍）； 3）检查增强效果 嗅皮层锥体细胞对传入神经刺激的反应 通往皮质（侧嗅道）的通路，用两种电测试 刺激和自然产生的气味。这项研究将提供 对理解嗅觉处理做出了重要贡献，从而有助于 理解和最终治疗嗅觉障碍。