Replication coupled DNA repair in trypanosomes - a model system for studying HelQ helicase.

该项目将涉及遗传学、生物化学和结构生物学的一系列核心实验技能。这些将通过生物信息学培训得到加强。将提供从火山盐铁中纯化表位标记的Hel308蛋白的生化技术培训，以及其DNA结合和加工活性的体外分析。后者将使用一系列合成DNA底物；Hel308与DNA及其伴侣蛋白的相互作用将通过尖端的生物物理技术（如微尺度热泳术）进行监测。我们非常需要能够在遗传学、生物化学和结构生物学的交叉领域有效工作的科学家。所提供的干湿实验室训练的结合将有助于满足这种跨学科科学的需求。

The project will involve a range of core experimental skills in genetics, biochemistry and structural biology. These will be augmented by training in bioinformatics.Training will be provided in biochemical techniques for the purification of epitope-tagged Hel308 protein from Haloferax volcanii, and in vitro analysis of its DNA binding and processing activities. The latter will use an array of synthetic DNA substrates; their interactions of Hel308 with DNA and with partner proteins will be monitored by cutting-edge biophysical techniques such as microscale thermophoresis.There is a great need for scientists who are able to work effectively at the interface between genetics, biochemistry and structural biology. The combination of wet and dry lab training provided will help address this need for inter-disciplinary science.