The role of hepatic epithelia in the resolution of human liver inflammation: translational approaches to inform drug discovery.

肝上皮在解决人类肝脏炎症中的作用：为药物发现提供信息的转化方法。

• 批准号: 2084576
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2084576
• 关键词: role; hepatic; epithelia; resolution; human

Pharmacological interventions can boost hepatocyte cell division and kick-start liver regeneration in patients with chronic liver diseases (CLD). Aims. We will study liver regeneration in vitro and ex vivo in human livers from patients with end stage diseases and in donor livers rejected for transplantation. We will use:i) high throughput imaging, measuring compound effects on hepatocyte viability, morphology, proliferation, apoptosis and ii) the validated cauterisation injury model (ZS), to establish the role of therapeutics in the context of human inflammation. iii) Quantitative and qualitative image analyses (UoB, UoN and AZ). Outcomes. The student will train with clinician and basic scientists to perform assays on human liver explant tissues in drug discovery, learning from experts at AZ and the universities of Birmingham and Nottingham.

药物干预可以促进慢性肝病（CLD）患者的肝细胞分裂并启动肝再生。目标。我们将在体外和离体研究终末期疾病患者的肝脏和移植排斥的供体肝脏的肝再生。我们将使用：用途：i）高通量成像，测量化合物对肝细胞活力、形态、增殖、凋亡的影响，和ii）经验证的烧灼损伤模型（ZS），以确定治疗剂在人类炎症背景下的作用。iii）定量和定性图像分析（UoB、UoN和AZ）。结果。学生将与临床医生和基础科学家一起培训，在药物发现中对人类肝脏外植体组织进行分析，向AZ以及伯明翰和诺丁汉大学的专家学习。