The role of hepatic epithelia in the resolution of human liver inflammation: translational approaches to inform drug discovery.

药物干预可以促进肝细胞分裂和启动慢性肝病（CLD）患者的肝脏再生。目标。我们将研究终末期疾病患者肝脏的体外和体外肝脏再生，以及因移植而被排斥的供体肝脏。我们将使用：i)高通量成像，测量对肝细胞活力、形态、增殖、凋亡的复合效应；ii)经过验证的烧灼损伤模型（ZS），以确定治疗方法在人类炎症背景下的作用。iii)定量和定性图像分析（UoB， UoN和AZ）。结果。该学生将与临床医生和基础科学家一起接受培训，向阿斯利康、伯明翰和诺丁汉大学的专家学习，对药物发现中的人类肝脏移植组织进行分析。

Pharmacological interventions can boost hepatocyte cell division and kick-start liver regeneration in patients with chronic liver diseases (CLD). Aims. We will study liver regeneration in vitro and ex vivo in human livers from patients with end stage diseases and in donor livers rejected for transplantation. We will use:i) high throughput imaging, measuring compound effects on hepatocyte viability, morphology, proliferation, apoptosis and ii) the validated cauterisation injury model (ZS), to establish the role of therapeutics in the context of human inflammation. iii) Quantitative and qualitative image analyses (UoB, UoN and AZ). Outcomes. The student will train with clinician and basic scientists to perform assays on human liver explant tissues in drug discovery, learning from experts at AZ and the universities of Birmingham and Nottingham.