Atmospheric Pressure Infrared MALDI Mass Spectrometry

大气压红外MALDI质谱法

• 批准号: 6486201
• 负责人: Vladimir M Doroshenko
• 金额: $ 62.01万
• 依托单位: SCIENCE AND ENGINEERING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6486201
• 关键词: atmospheric pressure; biomedical equipment development; electrospray ionization mass spectrometry; infrared spectrometry; ionization; lasers; matrix assisted laser desorption ionization; miniature biomedical equipment; physical separation; technology /technique development

The main purpose of this proposal is to develop a novel interface for on- line coupling of liquid separation techniques to a mass spectrometer (MS) using an atmospheric pressure (AP) infrared (IR) matrix-assisted laser desorption/ionization (MALDI) method. In contrast to conventional MALDI where ions are formed inside a vacuum system. In AP MALDI they are produced at normal atmospheric pressure. AP MALDI was first demonstrated and later commercialized using an ultraviolet (UV) laser. It showed sensitivity comparable to that of conventional MALDI, a large tolerance to the laser energy, and minimal fragmentation of analyte ions. AP MALDI interfaced with an ion trap mas spectrometer brings unique capability off tandem MS (or MS/MS) to MALDI analysis. Since AP UV-MALDI uses mostly solid organic matrices it like conventional UV- MALDI usually allows off-line analysis only. The use of an IR laser will allow one to use significantly larger number of matrices for MS analysis including liquid ones. Given excellent absorption or organic compounds in the IR spectral range the AP IR-MALDI makes possible direct coupling of liquid separation techniques =to MS, thus, the perspective of having both MALDI and electrospray ion sources for liquid analysis on the same mass analyzer becomes very feasible. PROPOSED COMMERCIAL APPLICATIONS: Atmospheric Pressure IR-MALDI for interfacing mass spectrometers to liquid separation techniques as well as for off-line analysis. This method has many biological and biomedical applications in research labs, hospitals, industry, and defense where analysis of proteins, peptides, DNA & RNA is utilized. Commercialization of the method by SESI is straightforward.

该方案的主要目的是开发一种新的接口，用于使用大气压红外(AP)红外(IR)基质辅助激光解吸/电离(MALDI)方法将液体分离技术在线耦合到质谱仪(MS)。与传统的MALDI不同，MALDI的离子是在真空系统中形成的。在AP MALDI中，它们是在常压下产生的。AP MALDI最初是用紫外线激光演示的，后来被商业化。它的灵敏度与传统的MALDI相当，对激光能量有很大的耐受性，分析物离子的碎裂最小。AP MALDI与离子陷阱质谱仪相结合，为MALDI分析带来了串联MS(或MS/MS)的独特能力。由于AP UV-MALDI主要使用固体有机基质，它与传统的UV-MALDI一样，通常只允许离线分析。红外激光的使用将使人们能够使用数量显著更多的基质进行MS分析，包括液体基质。由于在红外光谱范围内具有良好的吸收或有机化合物，AP IR-MALDI使液体分离技术与MS直接耦合成为可能，因此，在同一质量分析仪上同时使用MALDI和电喷雾离子源进行液体分析的前景变得非常可行。建议的商业应用：大气压IR-MALDI，用于质谱仪与液体分离技术的接口，以及用于离线分析。这种方法在研究实验室、医院、工业和国防中有许多生物和生物医学应用，其中使用蛋白质、多肽、DNA和RNA的分析。通过SESI将该方法商业化是很简单的。