IR MALDI ION TRAP MASS SPECTOMETER FOR DNA ANALYSIS

描述：（改编自申请人的摘要）质谱DNA 分析具有很大的潜力，因为它消除了DNA的耗时阶段 通过凝胶电泳和荧光分析的传统方法进行阅读。 提出了一种基质辅助激光解吸电离（MALDI）方法 离子阱质谱仪（ITMS）用于DNA分析。碎片的存在 在生物分子的MALDI光谱中非常常见， 简单的DNA混合物非常复杂，特别是当自动化系统 考虑了与飞行时间质谱仪（TOF）相比， 通常与MALDI离子源一起使用，该提议将提高 在MALDI过程中的分子DNA离子，增加灵敏度， 该方法的可靠性，并允许DNA MS分析的自动化。同相 I，一种ITMS，在低频下驱动，在高达m/z 30，000的范围内工作 将建立和接口与MALDI离子源，而不使用强大的 电场提取离子。可调谐红外激光器， 在获得具有最小碎片的质谱中的良好结果 TOF-MS的实验，将在MALDI离子源中使用。在第二阶段， 一个高通量DNA分析的自动化系统将使用 冷却的2D芯片样品阵列探针。 拟议商业应用：不可用

DESCRIPTION : (Adapted from that applicant's abstract) Mass spectrometric DNA analysis has a great potential since it eliminates time-consuming stages of DNA reading by traditional methods of gel electophoresis and fluorescent analysis . It is proposed to develop a matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer (ITMS) for DNA analysis. The presence of fragments is very common in MALDI spectra of biomolecules that makes analysis of even simple DNA mixtures extremely complicated especially when automated systems are considered. In contrast to the time-of-flight (TOF) mass spcetrometer (MS) commonly used with MALDI ion sources, this proposal will improve the yield of the molecular DNA ions in the MALDI process, increase the sensitivity and reliabilility of the method, and allow automation of DNA MS analysis. In Phase I, an ITMS driven at low frequency to operate in the range up to m/z 30,000 will be built and interfaced with MALDI ion source without using strong electric fields for extracting ions. A tunable infrared laser, which showed good results in obtaining mass spectra with minimum fragmentation in the experiments of TOF-MS, will be utilized in the MALDI ion source. In Phase II, an automated system for high throughput DNA analysis will be built using a cooled 2D chip sample array probe. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE