IR MALDI ION TRAP MASS SPECTOMETER FOR DNA ANALYSIS

描述：(改编自申请人的摘要)质谱学DNA 分析有很大的潜力，因为它消除了DNA的耗时阶段 读数采用传统的凝胶电泳法和荧光分析法。 提出了一种基于基质辅助的激光解吸电离(MALDI)技术 用于DNA分析的离子陷阱质谱仪(ITMS)。碎片的存在 在生物分子的MALDI光谱中非常常见，这使得分析偶数 简单的DNA混合物极其复杂，特别是当自动化系统 考虑过了。与飞行时间(TOF)质谱仪(MS)不同 通常与MALDI离子源一起使用，这一建议将提高 MALDI过程中的分子DNA离子，提高了灵敏度和 方法的可靠性，并允许DNA MS分析的自动化。同相 I，一种低频驱动的ITMS，工作在m/z 30,000的范围内 将被构建并与MALDI离子源接口，而无需使用强 用于提取离子的电场。一种可调谐的红外激光器，它显示了 在获得最小碎裂的质谱图方面取得了良好的结果 飞行时间质谱仪的实验，将用于MALDI离子源。在第二阶段， 一个用于高通量DNA分析的自动化系统将使用 冷却2D芯片样品阵列探头。 建议的商业应用：不可用

DESCRIPTION : (Adapted from that applicant's abstract) Mass spectrometric DNA analysis has a great potential since it eliminates time-consuming stages of DNA reading by traditional methods of gel electophoresis and fluorescent analysis . It is proposed to develop a matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer (ITMS) for DNA analysis. The presence of fragments is very common in MALDI spectra of biomolecules that makes analysis of even simple DNA mixtures extremely complicated especially when automated systems are considered. In contrast to the time-of-flight (TOF) mass spcetrometer (MS) commonly used with MALDI ion sources, this proposal will improve the yield of the molecular DNA ions in the MALDI process, increase the sensitivity and reliabilility of the method, and allow automation of DNA MS analysis. In Phase I, an ITMS driven at low frequency to operate in the range up to m/z 30,000 will be built and interfaced with MALDI ion source without using strong electric fields for extracting ions. A tunable infrared laser, which showed good results in obtaining mass spectra with minimum fragmentation in the experiments of TOF-MS, will be utilized in the MALDI ion source. In Phase II, an automated system for high throughput DNA analysis will be built using a cooled 2D chip sample array probe. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE