A Wire-ion-guide Fourier Transform Ion Cyclotron Resonance Mass Spectrometer For

描述（由申请人提供）：本项目旨在为傅里叶变换离子回旋共振质谱（FT ICR MS）引入一个新的方面，以改善传统FT ICR所实现的质量范围，同时保留FT ICR MS固有的高质量准确度，以扩展FT ICR方法用于生物研究和蛋白质组学的分析能力和能力。主要的修改涉及将中心线电极设计引入到ICR电池中，以实现可预测地导致ICR性能显著改善的电场修改。一个小型的台式FT ICR系统与1.2 T永磁体将被用作试验台的实施所提出的概念。新的ICR设计被称为线离子导向（WIG）ICR。WIG ICR MS将可预见地证明自下而上和自上而下蛋白质组学应用的质量范围和准确性的显着改进，这在类似条件下不可能与传统FTICR一起实现。蛋白质组学、糖组学和代谢组学工具的应用在现代生命科学、药物发现和临床应用中起着重要作用。我们提出了一种新的技术，用于提高生物分子鉴定和结构阐明的分析能力，提高质量范围，质量精度和分辨率的质谱分析，蛋白质组学，糖组学和代谢组学的一个重要战略今天。

DESCRIPTION (provided by applicant): This project is aimed at introducing a novel aspect to the Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) to improve the mass range beyond those achieved with traditional FT ICR while retaining a high mass accuracy inherent to FT ICR MS in order to extend the analytical power and the capabilities of the FT ICR method for biological research and proteomics. The major modification involves the introduction of a central wire electrode design into the ICR cell in order to achieve electric field modifications that predictably cause a significant improvement in the ICR performance. A small benchtop FT ICR system with a 1.2 T permanent magnet will be utilized as the test bed for the implementation of the proposed concepts. The new ICR design is called a wire-ion-guide (WIG) ICR. The WIG ICR MS will predictably demonstrate significant improvements in the mass range and accuracy for the bottom-up and top-down proteomics applications that are not possible with traditional FTICR under similar conditions. The application of proteomics, glycomics, and metabolomics tools plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new technology for increasing the analytical power of biomolecule identification and structure elucidation in terms of improved mass range, mass accuracy and resolution in mass spectrometry, an essential strategy in proteomics, glycomics, and metabolomics today.