A Wire-ion-guide Fourier Transform Ion Cyclotron Resonance Mass Spectrometer For

描述(申请人提供)：该项目旨在引入傅立叶变换离子回旋共振质谱仪(FT ICR MS)的一个新方面，以改进传统FT ICR MS获得的质量范围，同时保持FT ICR MS固有的高质量精度，以扩展FT ICR方法在生物研究和蛋白质组学方面的分析能力和能力。主要的改进包括在ICR电池中引入中心线电极设计，以实现可预测地导致ICR性能显著改善的电场修改。一个带有1.2T永磁体的小型台式FT ICR系统将被用作实施拟议概念的试验台。新的ICR设计被称为导线离子导引(WIG)ICR。可以预见，在自下而上和自上而下的蛋白质组学应用中，WIG ICR MS将显著改善质量范围和准确性，这是传统FTICR在类似条件下无法实现的。蛋白质组学、糖组学和代谢组学工具的应用在现代生命科学、药物开发和临床应用中发挥着重要作用。我们提出了一种新的技术来提高生物分子鉴定和结构鉴定的分析能力，这是当今蛋白质组学、糖组学和代谢组学中的一个基本策略，它改善了质谱学中的质量范围、质量精度和分辨率。

DESCRIPTION (provided by applicant): This project is aimed at introducing a novel aspect to the Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) to improve the mass range beyond those achieved with traditional FT ICR while retaining a high mass accuracy inherent to FT ICR MS in order to extend the analytical power and the capabilities of the FT ICR method for biological research and proteomics. The major modification involves the introduction of a central wire electrode design into the ICR cell in order to achieve electric field modifications that predictably cause a significant improvement in the ICR performance. A small benchtop FT ICR system with a 1.2 T permanent magnet will be utilized as the test bed for the implementation of the proposed concepts. The new ICR design is called a wire-ion-guide (WIG) ICR. The WIG ICR MS will predictably demonstrate significant improvements in the mass range and accuracy for the bottom-up and top-down proteomics applications that are not possible with traditional FTICR under similar conditions. The application of proteomics, glycomics, and metabolomics tools plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new technology for increasing the analytical power of biomolecule identification and structure elucidation in terms of improved mass range, mass accuracy and resolution in mass spectrometry, an essential strategy in proteomics, glycomics, and metabolomics today.