Quadrupole Ion Trap/FTICR Tandem Mass Spectrometer

四极杆离子阱/FTICR 串联质谱仪

• 批准号: 6831488
• 负责人: Vladimir M Doroshenko
• 金额: $ 10万
• 依托单位: SCIENCE AND ENGINEERING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2005-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6831488
• 关键词: biomedical equipment development; biotechnology; differential display technique; electrospray ionization mass spectrometry; gas chromatography mass spectrometry; high performance liquid chromatography; ion cyclotron resonance spectrometry; magnetic field; mass spectrometry; matrix assisted laser desorption ionization; method development; peptide structure; posttranslational modifications; protein sequence; proteomics

DESCRIPTION (provided by applicant): The purpose of this proposal is to develop a novel tandem mass spectrometry (or MS/MS) technology for mass and structural analysis of biomolecules that could combine excellent ion isolation and fragmentation capabilities of a quadrupole ion trap (QIT) and high resolution and mass accuracy of Fourier transform (FT) ion cyclotron resonance (ICR) mass spectrometry (MS). The main feature of this technology is the use of a permanent magnet for generation of magnetic field in ICR part of the proposed instrument that will make it cost-efficient, compact in size, and affordable for many applications. The major emphasis is made on producing a homogeneous magnetic field in a maximum space volume that to a certain extent can compensate limitations imposed by the use of a lower magnetic field generated by a permanent magnet in the ICR cell (about 0.6 Tesla compared to 7-12 Tesla generated by superconductive magnets). A new permanent magnet design is suggested to achieve this goal. The new instrument is expected to have size, cost and MS/MS capabilities of commercial QIT-MS systems and the resolving power and mass accuracy exceeding that of a popular hybrid quadrupole/time-of-flight (QqTOF) MS.

描述（由申请人提供）：本提案的目的是开发一种新的串联质谱（或MS/MS）技术，用于生物分子的质量和结构分析，该技术可以结合四极离子阱（QIT）出色的离子分离和碎片化能力以及傅里叶变换（FT）离子回旋共振（ICR）质谱（MS）的高分辨率和质量精度。该技术的主要特点是在拟议仪器的ICR部分使用永磁体产生磁场，这将使其具有成本效益，体积紧凑，并且适用于许多应用。主要的重点是在最大的空间体积内产生均匀的磁场，在一定程度上可以补偿ICR电池中使用永磁体产生的较低磁场所带来的限制（约0.6特斯拉，而超导磁体产生的磁场为7-12特斯拉）。提出了一种新的永磁设计方案来实现这一目标。新仪器预计具有商用QIT-MS系统的尺寸，成本和MS/MS能力，并且分辨率和质量精度超过流行的混合四极杆/飞行时间（QqTOF） MS。