Model for Electrode Testing and Reduced Animal Use

电极测试和减少动物使用的模型

• 批准号: 6484447
• 负责人: Stuart F Cogan
• 金额: $ 34.15万
• 依托单位: EIC LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6484447
• 关键词: alternatives to animals in research; animal tissue; biological models; biosensor device; cerebrospinal fluid; electrochemistry; electrodes; electronic recording system; electronic stimulator; embryonic stem cell; hyaluronate; model design /development; physical model

DESCRIPTION (provided by applicant): The development of in vitro models that provide useful, cost-effective tools for assessing long-term electrochemical function of chronically implanted electrodes is proposed. The in vitro models will provide an alternative to animal models for developing experimental protocols and assessing chronic electrode stability. They will allow long-term testing of electrochemical function and stability of electrodes under conditions that reflect the in vivo electrochemical environment without the expense and variability of conducting such testing in animals. The program focuses on electrodes for neural recording, functional electrical stimulation (FES), and chemical sensing in the central nervous system (CNS), although applications to electrodes in the periphery and to biostability of passive implants are anticipated. An inanimate model and a living model employing mouse progenitor cells are proposed. Both models are designed to mimic the compositional and diffusion environment of the CNS as it affects electrochemistry at an electrode. The inanimate model was developed in Phase I. The Phase II program will expand the model to include living cells. The validity of the inanimate and living models will be evaluated by comparison of electrode function in the in vitro models with that observed under identical test protocols in the chronic cat cortex.

描述(申请人提供)：体外模型的发展 提供有用的、经济高效的工具来评估长期电化学 提出了长期植入电极的作用。体外模型 将为开发实验动物模型提供一种替代方法 协议和评估慢性电极稳定性。他们将允许长期的 电极的电化学性能和稳定性测试 反映体内电化学环境的条件 在动物身上进行这种试验的费用和可变性。该计划 重点介绍用于神经记录、功能性电刺激的电极 (FES)和中枢神经系统(CNS)的化学感觉，尽管 在外围电极和无源生物稳定性方面的应用 植入物有望问世。一种无生命模型和一种使用老鼠的生物模型 提出了祖细胞的概念。这两种型号都是为了模仿 影响中枢神经系统的成分和扩散环境 在电极上的电化学。无生命模型是在第一阶段开发的。 第二阶段的计划将扩大模型，将活细胞包括在内。这个 无生命模型和活模型的有效性将通过比较 体外模型中的电极功能与在相同条件下观察到的 慢性猫皮质的测试方案。