Composite Supports for High Throughput Chromatography

高通量色谱复合支撑物

• 批准号: 6529837
• 负责人: RICHARD F HAMMEN
• 金额: $ 33.04万
• 依托单位: CHELATECH, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-15 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6529837
• 关键词: biomaterial development /preparation; carboxylate; chemical synthesis; chromatography; crosslink; high performance liquid chromatography; ion exchange chromatography; nucleic acid chemical synthesis; polymers; protein purification; proteomics; silicon compounds; technology /technique development

DESCRIPTION: (provide by applicant) Research and drug development in the pharmaceutical industry rely heavily upon separation science, particularly in this era of High Throughput genomic and proteomic analyses. Chromatographic methods used today separate proteins from other molecules by interactions with a solid porous support Solutes and solvent flowing through a chromatographic column of porous media prefer the path of least resistance and flow around the particles, rather than the tortuous pathway through the narrow pores where the functional chemistry is located. This leads to slow adsorption-desorption kinetics, band spreading, and loss of resolution. This Phase I Research was successful in literally turning this problem inside out, by synthesizing composite materials consisting of nonporous particles with "spider webs" of functional ligands crossing the interstitial spaces between the particles. The Specific Aims of this Research are to synthesize a series of composite chromatography columns with functional groups immobilized by extended tethers that place the functional molecule thousands of Angstroms from the surface of the solid support. Prototype products will be developed for the High Throughput Chromatography of a variety of proteins in both a column format and in a parallel plate format that will perform separations in a few seconds. PROPOSED COMMERCIAL APPLICATION: The composite materials developed in this Research will find commercial use for High Throughput Chromatographic separations and bioassays. Corporate partners will assist ChelaTech in evaluating the performance and the market competitiveness of the prototype products developed in Phase II. The sale and deployment of these products to the pharmaceutical and healthcare research industries will enable cost reductions, enhanced accuracy, and productivity increases in research to cure human disease.

描述：(申请人提供)研究和药物开发 制药行业严重依赖分离科学，特别是在 这个高通量基因组和蛋白质组分析的时代。色谱学 今天使用的方法通过相互作用将蛋白质从其他分子中分离出来 流经层析的固体多孔载体溶质和溶剂 多孔介质柱倾向于阻力最小的路径，并绕着 粒子，而不是通过狭窄的毛孔的曲折路径 功能化学位于。这会导致缓慢的吸附-解吸 动力学、频带扩展和分辨率损失。这项第一阶段的研究是 成功地彻底解决了这个问题，通过综合 由无孔颗粒和蜘蛛网组成的复合材料 功能配体穿过粒子之间的间隙。这个 本研究的具体目的是合成一系列复合材料 官能团由延伸系带固定的色谱柱 将功能分子从星体表面放置几千埃 坚实的支持。将开发适用于高吞吐量的原型产品 各种蛋白质的柱式和柱式层析 平行板格式，将在几秒钟内执行分离。 建议的商业应用： 本研究开发的复合材料将在高通量层析分离和生物检测中得到商业应用。企业合作伙伴将协助CheraTech评估在第二阶段开发的原型产品的性能和市场竞争力。向制药和医疗保健研究行业销售和部署这些产品将有助于降低成本、提高准确性和提高人类疾病研究的生产率。