COMPOSITE SUPPORTS FOR DNA SYNTHESIS AND CHROMATOGRAPHY

DNA 合成和色谱分析的复合支持

• 批准号: 2869988
• 负责人: RICHARD F HAMMEN
• 金额: $ 9.99万
• 依托单位: HAMMEN CORPORATION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-15 至 1999-11-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2869988
• 关键词: carboxylate; chemical synthesis; chromatography; crosslink; nucleic acid chemical synthesis; polymers; silicon compounds; technology /technique development

Research and drug development in the pharmaceutical industry rely upon synthetic chemistry and separation science. Oligonucleotide, peptide, and many combinatorial syntheses use solid-supported and automated synthetic methods. Analytical methods used today require the separation of analyte from other molecules, based upon interactions with a solid support. In these synthetic and analytical applications, there must be an interaction of reagents in solution with molecules attached to a solid material. The diffusion of a molecule from solution through the stagnant boundary layer at the solid-liquid interface to the immobilized molecule is a rate-limiting step. Boundary diffusion limits compromise the kinetics and efficiency of the solute's interaction with the immobilized species. The long-term objective of this research is to develop composite support materials that are not diffusion limited and will have unprecedented reaction and equilibration kinetics approaching those of solution phase processes. The Specific Aims of this Research are to synthesize a series of composite columns with functional groups immobilized by extended tethers that place the functional molecule thousands of Angstroms from the surface of the solid phase. The utility and kinetic characteristics of the tethered functional groups will be measured for the solid-phase synthesis of oligonucleotides and high speed chromatography of proteins. PROPOSED COMMERCIAL APPLICATIONS: The composite materials developed in this Research will find commercial use as products used for solid phase synthesis and for chromatographic separations and bioassay. The use of these products by the pharmaceutical and healthcare research industries will enable cost reductions, enhanced accuracy, and productivity increases in research to cure human disease.

制药工业的研究和药物开发依赖于合成化学和分离科学。寡核苷酸、肽和许多组合合成使用固相支持和自动化合成方法。今天使用的分析方法需要基于与固体支持物的相互作用将分析物与其他分子分离。在这些合成和分析应用中，溶液中的试剂必须与附着在固体材料上的分子相互作用。分子从溶液通过固液界面处的停滞边界层扩散到固定化分子是限速步骤。边界扩散限制妥协的动力学和效率的溶质的相互作用与固定化的物种。这项研究的长期目标是开发复合载体材料，不受扩散限制，将有前所未有的反应和平衡动力学接近那些溶液相过程。本研究的具体目的是合成一系列复合柱，其功能基团通过延长的栓系物固定，将功能分子置于离固相表面数千埃的位置。将测量拴系官能团的效用和动力学特性用于寡核苷酸的固相合成和蛋白质的高速色谱。拟议的商业应用：本研究开发的复合材料将作为固相合成、色谱分离和生物测定的产品进行商业应用。制药和医疗保健研究行业使用这些产品将能够降低成本，提高准确性，并提高治疗人类疾病的研究生产率。