COMPOSITE SUPPORTS FOR RAPID POLYNUCLEOTIDE SYNTHESIS

快速多核苷酸合成的复合支持物

• 批准号: 6294776
• 负责人: RICHARD F HAMMEN
• 金额: $ 10万
• 依托单位: CHELATECH, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2001-08-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6294776
• 关键词: chemical synthesis; high performance liquid chromatography; nucleic acid chemical synthesis; oligonucleotides; polymers; polynucleotides; technology /technique development

DESCRIPTION (applicant's abstract): Modern DNA synthesis methods make use of porous solid phase supports that are used to immobilize the first base in the sequence. This process is inherently inefficient, because solutes and solvent flowing through a column of porous media prefer the path of least resistance. Flow naturally goes around the particles, rather than through the tortuous pathway of the pores where the synthetic DNA is being made. This causes slow reaction kinetics and poor use of expensive reagents. A more serious problem is the fact that it is not feasible to synthesize long polynucleotides. The size constraints of the narrow pores make it impossible to prepare long DNA sequences in acceptable yields and purities. This Phase I Research proposes to literally turn this problem inside out, by synthesizing composite materials consisting of nonporous particles with spider webs of functional polymers crossing the interstitial spaces between the particles. The Aim of this Research is to attach nucleosides to the spider webs and synthesize DNA that will be positioned thousands of Angstroms from the surface of the solid support. Prototype products will be developed in Phase I, and tested for their yields of synthetic DNA production. PROPOSED COMMERCIAL APPLICATION: The composite materials developed in this Research will enable the high speed, low cost, production of both short and long DNA sequences. This will have a revolutionary effect in the entire biotechnology and pharmaceutical industries, and will reduce product development time and costs.

描述(申请人摘要)：现代DNA合成方法利用 多孔固相载体，用于将第一碱固定在 序列。这个过程本身效率很低，因为溶质和溶剂 流经多孔介质柱的流动更倾向于阻力最小的路径。 流动自然地绕过颗粒，而不是通过曲折的 制造合成DNA的毛孔的路径。这会导致速度缓慢 反应动力学和昂贵试剂的不良使用。一个更严重的问题是 事实上，合成长多核苷酸是不可行的。大小 狭孔的限制使得制备长DNA变得不可能 以可接受的产率和纯度测序。这项第一阶段的研究计划 通过合成复合材料，真正地颠覆了这个问题 由无孔颗粒与功能聚合物的蜘蛛网组成 穿过粒子之间的间隙。这样做的目的是 研究是将核苷连接到蜘蛛网上，并合成 将被放置在距离固体表面数千埃的地方 支持。原型产品将在第一阶段开发，并进行测试 合成DNA生产的产量。 建议的商业应用： 本研究开发的复合材料将使高速、低成本、 产生短DNA序列和长DNA序列。这将产生革命性的影响 在整个生物技术和制药行业，并将减少产品 开发时间和成本。