Histidine-Rich Glycoprotein: Characterization and Clinical Significance Studies

富含组氨酸的糖蛋白：表征和临床意义研究

• 批准号: 6103676
• 负责人: mcdonald k horne
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6103676
• 关键词: antifibrinolytic agents; blood proteins; chemical binding; enzyme linked immunosorbent assay; glycoproteins; histidine; human tissue; molecular site; oligosaccharides; plasminogen; protein isoforms; thrombosis

Histidine-rich glycoprotein (HRGP), a plasma protein with antiheparin and antifibrinolytic activity, has been associated with thrombophilia in three families. Therefore, it may promote hypercoagulability. The following objectives have been achieved in the laboratory: purification of the HRGP, using a combination of cobalt-sepharose, and development of an enzyme-linked immunosorbent assay for its quantitation. We have confirmed HRGP's antiheparin activity.In addition, we have demonstrated that HRGP binds to activated human platelets. The amount of binding is increased by several divalent cations, but not calcium or magnesium. It is also enhanced significantly by plasminogen, although HRGP does not have the reciprocal effect of increasing plasminogen binding to platelets. These observations suggest that platelet-bound plasminogen may serve as a binding site for HRGP, although there appear to be other sites as well.

富组氨酸糖蛋白（HRGP），一种血浆 具有抗肝素和抗纤维蛋白溶解活性的蛋白质， 与三个家族的血栓形成倾向有关。因此可能 促进高凝状态。以下目标已被 在实验室中实现：HRGP的纯化，使用 钴-琼脂糖凝胶的组合，以及开发 酶联免疫吸附测定用于其定量。我们有 证实了HRGP的抗肝素活性。此外，我们还 证明HRGP与活化的人血小板结合。的 结合量增加了几个二价阳离子，但不 钙或镁。它还大大增强了 纤维蛋白溶酶原，虽然HRGP没有相反的效果， 增加纤溶酶原与血小板的结合。这些观察结果 提示血小板结合纤溶酶原可能作为结合位点 对于HRGP，虽然似乎还有其他网站。