Using a combined approach of molecular microbiology, genomic mining and phylogenomics to discover and activate cryptic biosynthetic gene clusters in S

利用分子微生物学、基因组挖掘和系统基因组学的综合方法来发现和激活 S 中隐藏的生物合成基因簇

• 批准号: 2097313
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2097313
• 关键词: Using; combined; approach; molecular; microbiology

Actinobacteria are a type of soil bacteria and are a rich source of novel antimicrobials. Streptomyces is the largest group of bacteria in Actinobacteria and possess a number of discovered, undiscovered and putative cryptic gene clusters. Advances in deep sequencing technology, molecular cloning techniques and data mining has allowed the prediction of novel gene pathways through analysis and manipulation of regulatory factors in biosynthetic operons. This project will utilise a combined approach to target various soils to recover intact biosynthetic gene clusters and stimulate the expression of their cryptic products. Such processes will hopefully result in the discovery of novel natural compounds such as antibiotics and anticancer drugs.

放线菌是一类土壤细菌，是新型抗菌剂的丰富来源。链霉菌是放线菌中最大的一类细菌，具有许多已发现的、未发现的和推测的隐藏基因簇。深度测序技术、分子克隆技术和数据挖掘的进步使得能够通过分析和操纵生物合成操纵子中的调控因子来预测新的基因途径。该项目将利用一种组合方法来靶向各种土壤，以恢复完整的生物合成基因簇并刺激其隐藏产物的表达。这样的过程将有望发现新的天然化合物，如抗生素和抗癌药物。