DETERMINATION OF CHLORPHENIRAMINE IN HUMAN BLOOD SERUM

人血清中氯苯那敏的测定

• 批准号: 6101333
• 负责人: A V DEL GROSSO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6101333
• 关键词: analytical method; blood chemistry; chlorpheniramine; fluorescence; formates; gas chromatography mass spectrometry; glycoproteins; high performance liquid chromatography; method development

The determination of chlorpheniramine at low part per billion levels in blood serum is being developed to support a clinical study to be conducted under the direction of Paul Turkeltaub, M.D., Lab of Immunobiochemistry, DAPP, CBER. Extraction efficiency and injection volume are normalized by addition of tetradeuterated chlorpheniramine and monitoring of the analogous D4 fragment ion at m/e 207. Development of an alternate method, that would permit the resolution of chlorpheniramine into its enantiometic components, is in progress. HPLC columns based on alpha-glycoprotein and beta-cyclodextrin stationary phases have provided enantiomeric resolution. Work is in progress on a pre- column derivatization method involving reaction with benzylchloroformate, chiral HPLC and fluorescence detection that may provide the necessary sensitivity for detection.

氯苯那敏的检测方法 正在开发血清以支持将要进行的临床研究 在Paul Turkeltaub医学博士的指导下，免疫生物化学实验室， DAPP，CBER。 提取效率和进样体积通过下式标准化： 添加四氘代氯苯那敏并监测 类似的D4碎片离子，m/e 207。 开发替代方法， 这将允许扑尔敏拆分成其对映体， 组件，正在进行中。 基于α-糖蛋白的HPLC色谱柱， β-环糊精固定相提供了对映体拆分。 柱前衍生化方法的工作正在进行中， 与氯甲酸苄酯反应，手性HPLC和荧光检测 其可以提供用于检测的必要灵敏度。