DETERMINATION OF CHLORPHENIRAMINE IN HUMAN BLOOD SERUM

扑热息痛中低百万分之几的测定 目前正在开发血清以支持即将进行的临床研究。 在免疫生物化学实验室医学博士保罗·特克尔陶布的指导下， DAPP，CBER。萃取效率和进样量通过以下方法归一化 四氢扑热息痛的添加及监测 类似的D4碎片离子，m/e为207。开发一种替代方法， 这将允许扑尔敏拆分成其对映体 组件，正在进行中。基于α-糖蛋白和α-糖蛋白的高效液相色谱法 β-环糊精固定相提供了对映体拆分。 柱前衍生化方法的工作正在进行中，包括 氯甲酸苄酯反应、手性高效液相色谱和荧光检测 这可能为检测提供必要的敏感度。

The determination of chlorpheniramine at low part per billion levels in blood serum is being developed to support a clinical study to be conducted under the direction of Paul Turkeltaub, M.D., Lab of Immunobiochemistry, DAPP, CBER. Extraction efficiency and injection volume are normalized by addition of tetradeuterated chlorpheniramine and monitoring of the analogous D4 fragment ion at m/e 207. Development of an alternate method, that would permit the resolution of chlorpheniramine into its enantiometic components, is in progress. HPLC columns based on alpha-glycoprotein and beta-cyclodextrin stationary phases have provided enantiomeric resolution. Work is in progress on a pre- column derivatization method involving reaction with benzylchloroformate, chiral HPLC and fluorescence detection that may provide the necessary sensitivity for detection.