DETERMINATION OF URUSHIOL IN ALLERGENIC EXTRACTS--URUSHIOL STABILITY STUDIES

毒葛等植物致敏提取物中的活性成分 毒木属植物一般被命名为 “漆酚”是五烯基或十七烯基儿茶酚的混合物。 提取物 目前可用于口服给药或注射的制剂 在植物油中，例如杏仁油、玉米油或橄榄油。 漆酚化合物可以 氧化成能够进行迈克尔- 与亲核化合物的加成反应。 其他上的烯烃位点 漆酚分子可作为亲核试剂，导致 存在于溶液中的漆酚的低聚。 此项 项目是：a）制定方法， 漆酚，包括单个化合物的形态; B）开发 从植物油中分离漆酚的样品前处理技术 （c）制定方法，以确定氧化和 低聚漆酚降解产物; d）关联漆酚 降解与浓度、溶液类型和储存因素 条件 采用气相色谱法对漆酚样品进行了形态分析和定量 使用极性固定相的三甲基甲硅烷基衍生物色谱法 阶段OV-225 已对该方法进行了验证研究。 采用硅胶柱的正相HPLC法， 以及毛细管气相色谱法， 将提供单个化合物的更好的分辨率。 程序 漆酚的分离在高（大于100微克/毫升） 植物油中的浓缩物已经得到验证， 固相萃取法分离漆酚 超过10微克/毫升）的浓度。 初步研究 涉及通过凝胶渗透的漆酚低聚物的形态形成 已经进行了色谱分析。

The active constituents in allergenic extracts of Poison Ivy and other plants of the genus toxicodendron have been generically designated as "urushiol", a mixture of penta- or heptadecenyl catechols. Extracts currently available for oral administration or injection are formulated in plant oils, e.g. almond, corn or olive oil. Urushiol compounds may oxidize to orthoquinones which are capable of undergoing Michael- addition reactions with nucleophilic compounds. Olefinic sites on other urushiol molecules may function as nucleophiles, resulting in oligomerization of urushiols present in solution. Objectives of this project are: a) to develop methods for the quantitative analysis of urushiol, including speciation of individual compounds; b) to develop sample preparation techniques to isolate urushiol from plant oil matrices; c) to develop methodologies to determine oxidized and oligomerized urushiol degradation products; d) to correlate urushiol degradation with factors of concentration, solution type and storage conditions. Urushiol samples have been speciated and quantitated by gas chromatography of trimethylsilyl derivatives using a polar stationary phase, OV-225. Validation studies have been performed for this method. A normal phase HPLC method utilizing a silica column is to be investigated as well as a capillary gas chromatographic procedure that would provide better resolution of individual compounds. Procedures for the isolation of urushiol at high (greater than 100 microgram/ml) concentrations from plant oils have been validated, development of a solid phase extraction procedure for isolation of urushiol at low (less than 10 microgram/ml) concentrations is in progress. Initial studies involving speciation of urushiol oligomers by gel permeation chromatography have been performed.