DETERMINATION OF URUSHIOL IN ALLERGENIC EXTRACTS--URUSHIOL STABILITY STUDIES

致敏提取物中漆酚的测定--漆酚稳定性研究

• 批准号: 3811027
• 负责人: A V DEL GROSSO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3811027
• 关键词: allergens; analytical method; biological products; catechols; chemical stability; gas chromatography; gel filtration chromatography; high performance liquid chromatography; oxidation; plant extracts; plant poisons

The active constituents in allergenic extracts of Poison Ivy and other plants of the genus toxicodendron have been generically designated as "urushiol," a mixture of penta- or heptadecenyl catechols. Extracts currently available for oral administration or injection are formulated in plant oils, e.g. almond, corn or olive oil. Urushiol compounds may oxidize to orthoquinones which are capable of undergoing Michael-addition reactions with nucleophilic compounds. Olefinic sites on other urushiol molecules may function as nucleophiles, resulting in oligomerization of urushiols present in solution. Objectives of this project are: a) to develop methods for the quantitative analysis of urushiol, including speciation of individual compounds; b) to develop sample preparation techniques to isolate urushiol from plant oil matrices; c) to develop methodologies to determine oxidized and oligomerized urushiol degradation products; d) to correlate urushiol degradation with factors of concentration, solution type and storage conditions. Urushiol samples have been speciated and quantitated by gas chromatography of trimethylsilyl derivatives using a polar stationary phase, OV-225. Validation studies have been performed for this method. A normal phase HPLC method utilizing a silica column is to be investigated. Procedures for the isolation of urushiol at high (greater than 100 pg/ml) concentrations from plant oils have been validated, development of a solid phase extraction procedure for isolation of urushiol at low (less than 10 pg/ml) concentrations is in progress. Initial studies involving speciation of urushiol oligomers by gel permeation chromatography have been performed.

毒葛等植物致敏提取物中的活性成分 毒木属植物一般被命名为 “漆酚”，五烯基或十七烯基儿茶酚的混合物。提取物 目前可用于口服给药或注射的制剂 植物油，例如杏仁油、玉米油或橄榄油。漆酚化合物可氧化 到能够进行迈克尔加成反应的邻醌 亲核化合物。其它漆酚分子上的烯烃位点可 作为亲核试剂，导致存在的漆酚的低聚 在溶液中。该项目的目标是：（a）制定 漆酚的定量分析，包括个体形态分析 B）开发分离漆酚的样品制备技术 c）开发方法学，以确定氧化的 和低聚漆酚降解产物; d）将漆酚 降解与浓度、溶液类型和储存因素 条件采用气相色谱法对漆酚样品进行了形态分析和定量 使用极性固定相的三甲基甲硅烷基衍生物色谱法 阶段OV-225已对该方法进行了验证研究。一 正相高效液相色谱法使用硅胶柱进行研究。 用于分离高浓度漆酚（大于100 pg/ml）的方法 从植物油的浓度已经得到验证，固体的发展 在较低（小于10 pg/ml）的浓度。涉及物种形成的初步研究 漆酚低聚物的凝胶渗透色谱法。