DIAMOND MICROELECTRODES FOR NEUROTRANSMITTER DETECTION

用于神经递质检测的金刚石微电极

• 批准号: 6531014
• 负责人: HEIDI B MARTIN
• 金额: $ 4.42万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6531014
• 关键词: biomaterial compatibility; biomaterial development /preparation; biomaterial evaluation; biomaterial interface interaction; biosensor device; dopamine; electrophysiology; enzyme activity; extracellular; implant; laboratory rat; medical implant science; microelectrodes; neurotransmitters; oxidation reduction reaction; single cell analysis; surface coating; tissue /cell culture

Boron-doped diamond film microelectrode will be fabricated, characterized and tested as a novel sensor for neurotransmitters. The remarkable properties of the newly developed diamond electrodes are ideal for biochemical sensing applications, where long term stability, species selectivity, and high sensitivity are required. Diamond is just beginning to be explored as a material for biomedical applications. Polycrystalline and single-crystal diamond coatings will be vapor deposited onto substrates including carbon microfibers, and extensive materials characterization made. Surface modification of diamond electrodes will be performed. The electrochemical response as a function of the diamond film quality, crystallinity, sp2 content, and surface termination will be tested. Initially, dopamine oxidation kinetics, temporal response, and sensitivity will be measured. The surface termination and functionalization of polycrystalline and single- crystal diamond electrodes will be engineered to address issues of sensitivity and selectivity of, e.g., dopamine in the presence of ascorbic acid. Single-cell, in vitro, and in vivo experiments will be conducted and the diamond electrode stability measured. Spatial variations and drug effects will be monitored. Diamond's lower baseline current should make measurement of basal neurotransmission possible. For the first time, long-term in vivo experiments will be possible. Surface modification will allow studies of enzyme-mediated reactions. With diamond's attractive electrochemical properties and well-established fabrication techniques, this project could lead to new applications of diamond film microelectrodes for long-term, in vivo biosensors.

硼掺杂金刚石膜微电极将作为一种新型的神经递质传感器进行制备、表征和测试。新开发的金刚石电极的显著性能是生化传感应用的理想选择，这些应用需要长期稳定性、物种选择性和高灵敏度。钻石作为生物医学应用材料的探索才刚刚开始。多晶和单晶金刚石涂层将被气相沉积到包括碳微纤维在内的基材上，并进行广泛的材料表征。将对金刚石电极进行表面改性。将测试作为金刚石膜质量、结晶度、sp2含量和表面终止性的函数的电化学响应。首先，将测量多巴胺氧化动力学、时间响应和灵敏度。将设计多晶和单晶金刚石电极的表面终止和功能化，以解决例如在抗坏血酸存在的情况下多巴胺的敏感性和选择性问题。将进行单细胞、体外和体内实验，并测量金刚石电极的稳定性。将监测空间差异和药物效应。钻石较低的基线电流应该使测量基础神经传递成为可能。第一次，长期的体内实验将成为可能。表面修饰将使酶介导的反应研究成为可能。凭借金刚石诱人的电化学性质和成熟的制造技术，该项目可能会导致金刚石薄膜微电极在长期、在体生物传感器中的新应用。