DIAMOND MICROELECTRODES FOR NEUROTRANSMITTER DETECTION

用于神经递质检测的金刚石微电极

• 批准号: 6071316
• 负责人: HEIDI B MARTIN
• 金额: $ 3.03万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6071316
• 关键词: biomaterial compatibility; biomaterial development /preparation; biomaterial evaluation; biomaterial interface interaction; biosensor device; dopamine; electrophysiology; enzyme activity; extracellular; implant; laboratory rat; medical implant science; microelectrodes; neurotransmitters; oxidation reduction reaction; single cell analysis; surface coating; tissue /cell culture

Boron-doped diamond film microelectrode will be fabricated, characterized and tested as a novel sensor for neurotransmitters. The remarkable properties of the newly developed diamond electrodes are ideal for biochemical sensing applications, where long term stability, species selectivity, and high sensitivity are required. Diamond is just beginning to be explored as a material for biomedical applications. Polycrystalline and single-crystal diamond coatings will be vapor deposited onto substrates including carbon microfibers, and extensive materials characterization made. Surface modification of diamond electrodes will be performed. The electrochemical response as a function of the diamond film quality, crystallinity, sp2 content, and surface termination will be tested. Initially, dopamine oxidation kinetics, temporal response, and sensitivity will be measured. The surface termination and functionalization of polycrystalline and single- crystal diamond electrodes will be engineered to address issues of sensitivity and selectivity of, e.g., dopamine in the presence of ascorbic acid. Single-cell, in vitro, and in vivo experiments will be conducted and the diamond electrode stability measured. Spatial variations and drug effects will be monitored. Diamond's lower baseline current should make measurement of basal neurotransmission possible. For the first time, long-term in vivo experiments will be possible. Surface modification will allow studies of enzyme-mediated reactions. With diamond's attractive electrochemical properties and well-established fabrication techniques, this project could lead to new applications of diamond film microelectrodes for long-term, in vivo biosensors.

硼掺杂金刚石薄膜微电极将作为一种新型的神经递质传感器进行制备、表征和测试。新开发的金刚石电极的显著特性是理想的生化传感应用，在长期稳定性，物种选择性和高灵敏度的要求。金刚石作为生物医学材料的应用才刚刚开始被探索。多晶和单晶金刚石涂层将气相沉积在基材上，包括碳微纤维，并进行广泛的材料表征。对金刚石电极进行表面改性。电化学响应作为金刚石膜质量、结晶度、sp2含量和表面终止的函数将被测试。最初，多巴胺氧化动力学，时间反应和敏感性将被测量。多晶和单晶金刚石电极的表面终止和功能化将被设计来解决敏感性和选择性问题，例如，在抗坏血酸存在下多巴胺。将进行单细胞、体外和体内实验，并测量金刚石电极的稳定性。将监测空间变化和药物效应。戴蒙德较低的基线电流应该可以测量基础神经传递。长期的体内实验将首次成为可能。表面修饰将允许研究酶介导的反应。由于金刚石具有吸引人的电化学特性和完善的制造技术，该项目可能会为长期的体内生物传感器带来金刚石膜微电极的新应用。