Microwave-Enhanced Luminescence to Detect Growth Factors

微波增强发光检测生长因子

• 批准号: 6548580
• 负责人: MARK T. MARTIN
• 金额: $ 14.81万
• 依托单位: MIRARI BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6548580
• 关键词: angiogenesis factor; biological signal transduction; biomedical equipment development; growth factor; immunologic assay /test; luminescence; messenger RNA; microprocessor /microchip; microwave radiation; polymerase chain reaction; technology /technique development

DESCRIPTION (provided by applicant): "Microwave Chemiluminescence"(MCL) is a broad new enabling technology that promises to greatly improve the speed and convenience of numerous types of chemiluminescence (CL)-based analyses. MCL is the first use of targeted microwave chemistry in the field of bioanalyses. Microchip-based MCL assays combine the exquisite specificity of antibodies or hybridized nucleic acids with the focused heat energy of microwave radiation. Microwaves raise the temperature of the microchip surface, initiating and greatly accelerating the rates of surface-bound CL reporter reactions. MCL instrumentation and chips will be developed for the overall goal of increasing the productivity of cancer research. In Phase I, we will demonstrate the technology using TNFalpha as the first immunoassay analyte. Further research, Phases II and III, will focus on multi-analyte panels measuring angiogenic growth factors. Studies of angiogenesis are critical to the understanding and treatment of solid tumors and such panels using MCL detection will fill an unmet need in cancer research laboratories. Moreover, an angiogenesis factor panel is a logical entry point to our long-term goal of greatly expanded arrays of cancer-related analytes, including endogenous angiogenesis inhibitors, growth factor receptors, and signal transduction proteins. PROPOSED COMMERCIAL APPLICATION: The overall research objective is to develop a new analytical technology that will assist cancer researchers by bringing the sensitivity chemiluminescence sensitivity to a convenient microchip array format. A panel of chip-based immunoassays for angiogenesis factors will be developed to assist basic and clinical researchers in cancer research and drug discovery. Future work will expand the technology to other applications in protein and nucleic acid analysis.

描述(由申请人提供)： “微波化学发光”(MCL)是一种广泛的新使能技术 承诺将极大地提高各种类型的 基于化学发光(CL)的分析。MCL是第一个使用目标的 生物分析领域中的微波化学。基于微芯片的MCL分析 结合了抗体或杂交核酸的精致特异性 利用微波辐射的聚焦热能。微波提高了 微芯片表面的温度，引发并极大地加速 表面结合化学发光报告反应的速率。MCL仪器和芯片 将为提高工作效率的总体目标而开发 癌症研究。在第一阶段，我们将使用TNFpha演示该技术 作为第一个免疫分析分析物。进一步的研究，第二阶段和第三阶段，将 专注于多分析小组测量血管生成生长因子。的研究 血管生成对实体瘤的认识和治疗至关重要 这种使用MCL检测的面板将填补癌症研究中未得到满足的需求 实验室。此外，血管生成因子小组是一个合乎逻辑的切入点。 我们的长期目标是大幅扩大癌症相关分析物的阵列， 包括内源性血管生成抑制物、生长因子受体和 信号转导蛋白。 拟议的商业应用：总体研究目标是开发一种新的分析技术，通过将灵敏度化学发光灵敏度转化为方便的微芯片阵列格式来帮助癌症研究人员。将开发一组基于芯片的血管生成因子免疫分析方法，以帮助基础和临床研究人员进行癌症研究和药物发现。未来的工作将把这项技术扩展到蛋白质和核酸分析的其他应用。