Microwave-Enhanced Luminescence to Detect Growth Factors

描述（由申请人提供）： “微波化学发光”（MCL）是一种广泛的新的使能技术， 承诺大大提高速度和方便的许多类型的 化学发光（CL）分析。MCL是第一次使用靶向 微波化学在生物分析领域的应用基于微芯片的MCL测定 联合收割机将抗体或杂交核酸的精确特异性 微波辐射的集中热能。微波炉提高了 微芯片表面的温度，引发并大大加速了 表面结合CL报告反应的速率。MCL仪器和芯片 将为提高生产力的总体目标而开发， 癌症研究。在第一阶段，我们将展示使用TNF α的技术， 作为第一免疫测定分析物。第二和第三阶段的进一步研究将 专注于测量血管生成生长因子的多分析物面板。研究 血管生成对于理解和治疗实体瘤至关重要 这种使用MCL检测的面板将填补癌症研究中未满足的需求 laboratories.此外，血管生成因子面板是一个合乎逻辑的切入点 对于我们大大扩展癌症相关分析物阵列的长期目标， 包括内源性血管生成抑制剂、生长因子受体， 信号转导蛋白 拟定商业应用：总体研究目标是开发一种新的分析技术，通过将灵敏度化学发光灵敏度引入方便的微芯片阵列格式来帮助癌症研究人员。 将开发一组基于芯片的血管生成因子免疫测定，以协助癌症研究和药物发现的基础和临床研究人员。 未来的工作将把这项技术扩展到蛋白质和核酸分析的其他应用。

DESCRIPTION (provided by applicant): "Microwave Chemiluminescence"(MCL) is a broad new enabling technology that promises to greatly improve the speed and convenience of numerous types of chemiluminescence (CL)-based analyses. MCL is the first use of targeted microwave chemistry in the field of bioanalyses. Microchip-based MCL assays combine the exquisite specificity of antibodies or hybridized nucleic acids with the focused heat energy of microwave radiation. Microwaves raise the temperature of the microchip surface, initiating and greatly accelerating the rates of surface-bound CL reporter reactions. MCL instrumentation and chips will be developed for the overall goal of increasing the productivity of cancer research. In Phase I, we will demonstrate the technology using TNFalpha as the first immunoassay analyte. Further research, Phases II and III, will focus on multi-analyte panels measuring angiogenic growth factors. Studies of angiogenesis are critical to the understanding and treatment of solid tumors and such panels using MCL detection will fill an unmet need in cancer research laboratories. Moreover, an angiogenesis factor panel is a logical entry point to our long-term goal of greatly expanded arrays of cancer-related analytes, including endogenous angiogenesis inhibitors, growth factor receptors, and signal transduction proteins. PROPOSED COMMERCIAL APPLICATION: The overall research objective is to develop a new analytical technology that will assist cancer researchers by bringing the sensitivity chemiluminescence sensitivity to a convenient microchip array format. A panel of chip-based immunoassays for angiogenesis factors will be developed to assist basic and clinical researchers in cancer research and drug discovery. Future work will expand the technology to other applications in protein and nucleic acid analysis.