Evaluation of a Device Designed for Microwave Accelerated Protein Chip Assays

微波加速蛋白质芯片检测装置的评估

• 批准号: 7051057
• 负责人: MARK T. MARTIN
• 金额: $ 19.99万
• 依托单位: MIRARI BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7051057
• 关键词: biotechnology; cytokine; immunologic assay /test; luminescence; microwave radiation; proteomics; technology /technique development

DESCRIPTION (provided by applicant): Project Summary: The explosive growth of proteomics has increased the demand for rapid and convenient means of studying biomolecular binding interactions. Our goal in this project is to build and test a novel device for a new immunoassay technology called Microwave-Accelerated Surface Chemistry (MASC). MASC consists of two components; preparative, which involves accelerating antibody binding events, and analytical, which involves microwave-triggered reporter chemiluminescence. The device will be, in essence, a microwave incubator/luminometer for performing chip-based immunoassays. MASC chips are unique in containing microwave-active dielectric materials. When irradiated by low power microwaves, the chips undergo controlled temperature increases, inducing preparative and analytical surface reactions. The device will consist of a microwave source (magnetron), a novel waveguide-based reaction cell, a photomultiplier tube to capture light emitted from chips, and a personal computer to initiate reactions and collect and analyze data. By performing chip reactions within a waveguide, we will obtain much more uniform and controllable heating than the current use of a microwave oven cavity. This innovation will confer leapfrog advances in data quality and throughput. If successful, this waveguide-based incubator/luminometer will directly lead to commercial MASC systems. Our first aim in the research is to build a high quality device according to a pre-existing design. Our second and third aims are to achieve specific performance improvements over conventional control methods in rapid antibody binding and microwave chemiluminescence, respectively. We will use a cytokine panel for prototype chip immunoassays. We plan to commercialize MASC devices and chips for immunoassay-based research. The products will accelerate drug discovery and development and reduce associated costs. Relevance to Public Health: Our goal is to dramatically shrink the time required to perform bioanalytical tests called immunoassays using a new technology called Microwave-Accelerated Surface Chemistry (MASC). By reducing immunoassay times from hours to minutes, MASC will accelerate the discovery and development of drugs from proteomics research. The remarkable speed of MASC also holds promise for future rapid immunoassay-based clinical diagnostics and field testing.

描述（由申请人提供）： 项目摘要：蛋白质组学的爆炸式增长增加了对快速、便捷的生物分子结合相互作用研究方法的需求。我们在这个项目中的目标是构建和测试一种用于新型免疫分析技术的新型设备，称为微波加速表面化学（MASC）。 MASC 由两个部分组成；制备型涉及加速抗体结合事件，分析型涉及微波触发报告化学发光。该设备本质上是一个用于执行基于芯片的免疫测定的微波培养箱/光度计。 MASC 芯片的独特之处在于含有微波活性介电材料。当受到低功率微波照射时，芯片会经历受控的温度升高，从而引发制备和分析表面反应。该设备将由微波源（磁控管）、新型波导反应池、捕获芯片发出的光的光电倍增管以及启动反应并收集和分析数据的个人计算机组成。通过在波导内进行芯片反应，我们将获得比目前使用的微波炉腔体更加均匀和可控的加热。这项创新将带来数据质量和吞吐量的跨越式进步。如果成功，这种基于波导的培养箱/发光计将直接导致商业 MASC 系统。我们研究的首要目标是根据现有设计构建高质量的设备。我们的第二个和第三个目标是分别在快速抗体结合和微波化学发光方面实现比传统控制方法具体的性能改进。我们将使用细胞因子组进行原型芯片免疫测定。我们计划将 MASC 设备和芯片商业化，用于基于免疫分析的研究。这些产品将加速药物发现和开发并降低相关成本。与公共卫生的相关性：我们的目标是使用微波加速表面化学 (MASC) 新技术，大幅缩短执行称为免疫测定的生物分析测试所需的时间。通过将免疫测定时间从几小时缩短至几分钟，MASC 将加速蛋白质组学研究中药物的发现和开发。 MASC 的惊人速度也为未来基于快速免疫分析的临床诊断和现场测试带来了希望。