Continuous Digital Video Behavioral Analysis of Rats

描述（由申请人提供）：啮齿动物，主要是大鼠和小鼠的电图癫痫发作的行为成分通常被用作癫痫发作存在的最基本依据。许多研究基金将动物癫痫分析作为研究的基本组成部分，甚至是针对分子和细胞机制的研究。运动癫痫发作期间的异常行为通常是独特的，但从一个实验室到另一个实验室都存在一定程度的主观性。随着用于多次记录的复杂电极的发展和无线电遥测系统的可用性，将癫痫发作行为和电图癫痫发作活动的自动化研究结合起来的潜力现在是可能的。我们建议使用数字视频技术开发和验证行为算法，用于识别、分类和量化小鼠和大鼠不同类型癫痫发作的运动成分。我们将定义不同类型癫痫发作的行为成分，并将其与脑电图记录的电图癫痫发作相结合。这些行为将包括但不限于单侧和双侧前肢冠状，并假设前凸姿势，具有尾巴的特征定位。为了满足大学和制药公司的研究实验室的需求，将充分开发一种允许脑电图和行为结合分析的系统。

DESCRIPTION (provided by applicant): The behavioral components of electrographic seizures in rodents, primarily rats and mice, are generally used as the most fundamental basis for the presence of epileptic seizures. Numerous research grants use analysis of animal seizures as a fundamental component of the research, even research aimed at molecular and cellular mechanisms. The abnormal behaviors during a motor seizure are generally distinctive, but a certain degree of subjectivity is present from one laboratory to another. With the development of sophisticated electrodes for multiple recordings and the availability of radiotelemetry systems, the potential to combine automated studies of seizure behavior and electrographic seizure activity is now possible. We propose to develop and validate behavioral algorithms for identifying, classifying and quantifying the motor components of different types of seizures in mice and rats using digital video technology. We will define the behavioral components of different classes of seizures, and to do this in conjunction with electrographic seizures from EEG recordings. These behaviors will include but not be limited to unilateral and bilateral forelimb clonus and the assumption of a lordotic posture, with a characteristic positioning of the tail. A system that allows combined analysis of EEG and behavior will be fully developed to meet the demand from research laboratories at universities and pharmaceutical companies.