A Novel Sensor for total Mercury in Fish Tissue

鱼类组织中总汞的新型传感器

• 批准号: 6485022
• 负责人: SHANTHA s SARANGAPANI
• 金额: $ 9.99万
• 依托单位: INNOVATIVE CHEMICAL/ENVIRONMENTAL TECH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-15 至 2003-11-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485022
• 关键词: biomedical equipment development; biosensor device; electrodes; environmental contamination; environmental toxicology; fish; food contamination; food science /technology; mercury; mercury poisoning; monitoring device; portable biomedical equipment; seafood poisoning

DESCRIPTION (Applicant's abstract): There is a current need to monitor food and fish tissue samples for total mercury and other toxic metals, onsite, using portable instruments. Currently the determination of mercury requires complex instrumentation, which is beyond the means of potential users. Moreover, the samples have to be shipped to an accredited lab with typical turnaround times of 10 days or more. The fish tissue sample preparation involves a tedious procedure of hot, concentrated, acid digestion. This Phase I research will examine the feasibility of using screen-printed electrodes with dendrimer-gold nano composite for the "in situ" anodic stripping voltammetric analysis (ASV) of total mercury in fish tissue samples. Alternate methods of sample preparations will be tested. The dendrimeric structure due to its hydrophilicity and density of the molecular arms are expected to prevent the influx of large organic and biomolecules. With the advent of nano scale molecular reservoirs such as the dendrimers, and excellent opportunity exists to test the idea of rapid chemical sensing in a biomimetric manner. The screen-printed electrodes will be fabricated at the New Mexico State University Microfabrication Labs using our proprietary formulations. A thorough characterization of the proposed electrode, using certified fish tissue samples for "proof of the concept" will be carried out. The product from this research would be well suited for portable automated commercial instruments such as the MetalyzerTM, SA-5000 or Tracelab TM systems using the current EPA protocols for mercury analysis by stripping methods. The cost of the proposed electrode strips is comparable to the current cost of electrodes. The ASV has remarkably low detection limits and can analyze a variety of toxic metals due to the unique stripping potentials for each metal. PROPOSED COMMERCIAL APPLICATION: The proposed electrodes strips have a massive potential for use in field tests for agriculture and food industry. Water quality as well as for routine monitoring of industrial waste water treatment facilities to monitor low levels of toxic mercury and other medals. Multianalyte sensing capabilities could be incorporated. The US and European market for the proposed sensors is projected to be close to 400 million dollars by the year 2005.

描述（申请人摘要）：目前需要监测食品和 现场使用鱼组织样本检测汞和其他有毒金属总量 便携式仪器目前，汞的测定需要复杂的 仪器，这超出了潜在用户的能力。而且 样品必须运送到具有典型周转时间的认证实验室 10天或以上。鱼组织样品制备涉及繁琐的 热的、浓缩的、酸消化的过程。 第一阶段的研究将探讨使用丝网印刷的可行性， 树枝状大分子-金纳米复合物"原位"阳极电极 鱼组织样品中总汞的溶出伏安分析。 将检测样品制备的替代方法。树枝状聚合物 由于其亲水性和分子臂的密度， 预计可以防止大的有机分子和生物分子的流入。与 纳米级分子储库如树枝状聚合物的出现，以及优异的 有机会在生物测量中测试快速化学传感的想法 方式丝网印刷电极将在新墨西哥州制造 州立大学微加工实验室使用我们的专有配方。一 使用经认证的fish对拟定电极进行全面表征 将进行组织样本"概念验证"。的产物 这项研究将非常适合于便携式自动商业 仪器，如MetalyzerTM，SA-5000或TracelabTM系统， 目前EPA的汞分析方案，采用溶出法。的成本 所提出的电极条与电极的当前成本相当。 ASV具有非常低的检测限，可以分析各种有毒物质。 由于每种金属独特的剥离电位， 拟定商业应用： 所提出的电极条在现场测试中具有巨大的潜力 用于农业和食品工业。水质以及常规 监测工业废水处理设施，以监测低水平 有毒的水银和其他奖章多分析物传感能力可以是 incorporated.预计美国和欧洲市场的拟议传感器 到2005年将接近4亿美元。