A Novel Sensor for total Mercury in Fish Tissue

鱼类组织中总汞的新型传感器

• 批准号: 6485022
• 负责人: SHANTHA s SARANGAPANI
• 金额: $ 9.99万
• 依托单位: INNOVATIVE CHEMICAL/ENVIRONMENTAL TECH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-15 至 2003-11-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485022
• 关键词: biomedical equipment development; biosensor device; electrodes; environmental contamination; environmental toxicology; fish; food contamination; food science /technology; mercury; mercury poisoning; monitoring device; portable biomedical equipment; seafood poisoning

DESCRIPTION (Applicant's abstract): There is a current need to monitor food and fish tissue samples for total mercury and other toxic metals, onsite, using portable instruments. Currently the determination of mercury requires complex instrumentation, which is beyond the means of potential users. Moreover, the samples have to be shipped to an accredited lab with typical turnaround times of 10 days or more. The fish tissue sample preparation involves a tedious procedure of hot, concentrated, acid digestion. This Phase I research will examine the feasibility of using screen-printed electrodes with dendrimer-gold nano composite for the "in situ" anodic stripping voltammetric analysis (ASV) of total mercury in fish tissue samples. Alternate methods of sample preparations will be tested. The dendrimeric structure due to its hydrophilicity and density of the molecular arms are expected to prevent the influx of large organic and biomolecules. With the advent of nano scale molecular reservoirs such as the dendrimers, and excellent opportunity exists to test the idea of rapid chemical sensing in a biomimetric manner. The screen-printed electrodes will be fabricated at the New Mexico State University Microfabrication Labs using our proprietary formulations. A thorough characterization of the proposed electrode, using certified fish tissue samples for "proof of the concept" will be carried out. The product from this research would be well suited for portable automated commercial instruments such as the MetalyzerTM, SA-5000 or Tracelab TM systems using the current EPA protocols for mercury analysis by stripping methods. The cost of the proposed electrode strips is comparable to the current cost of electrodes. The ASV has remarkably low detection limits and can analyze a variety of toxic metals due to the unique stripping potentials for each metal. PROPOSED COMMERCIAL APPLICATION: The proposed electrodes strips have a massive potential for use in field tests for agriculture and food industry. Water quality as well as for routine monitoring of industrial waste water treatment facilities to monitor low levels of toxic mercury and other medals. Multianalyte sensing capabilities could be incorporated. The US and European market for the proposed sensors is projected to be close to 400 million dollars by the year 2005.

描述（申请人的摘要）：目前需要监视食物和 使用汞总金属和其他有毒金属的鱼组织样品，现场使用 便携式乐器。目前的汞的确定需要复杂 仪器，这超出了潜在用户的手段。而且， 样品必须运送到具有典型周转时间的认可实验室 10天或更长时间。鱼组织样品制备涉及繁琐的 热，浓缩，酸消化的程序。 本阶段I研究将检查使用屏幕打印的可行性 带有树枝状聚合物纳米复合材料的电极，用于“原位”阳极 鱼组织样品中总汞的伏安分析（ASV）。 样品制剂的替代方法将进行测试。树枝状大分子 由于其亲水性和分子臂密度为 预计可以防止大量有机和生物分子的流入。与 纳米秤的出现分子储层，例如树枝状聚合物，优秀 存在机会测试仿生中快速化学传感的想法 方式。屏幕打印的电极将在新墨西哥州制造 州立大学微加工实验室使用我们的专有配方。一个 使用经过认证的鱼对拟议的电极的透彻表征 将进行“概念证明”的组织样品。产品来自 这项研究非常适合便携式自动广告 诸如Metalyzertm，SA-5000或Tracelab TM系统等仪器使用 通过剥离方法进行汞分析的当前EPA方案。费用 提出的电极条与电极的当前成本相当。 ASV的检测极限非常低，可以分析各种有毒 金属由于每种金属具有独特的剥离电势。 拟议的商业应用： 提出的电极条具有在现场测试中使用的巨大潜力 农业和食品工业。水质和常规 监视工业废水处理设施以监测低水平 有毒的汞和其他奖牌。多元分析物传感功能可能是 并入。预计美国和欧洲的传感器市场 到2005年将接近4亿美元。