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Molecular Pathobiology of Langerhans Cell Histiocytosis

朗格汉斯细胞组织细胞增多症的分子病理学

基本信息

批准号：
6471618
负责人：
Barrett J. Rollins
金额：
$ 31.97万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-04-01 至 2003-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6471618
关键词：
Langerhans' cell chemokine cytokine receptors human tissue immunocytochemistry laboratory mouse laboratory rabbit leukocytosis molecular pathology nucleic acid hybridization patient oriented research receptor expression reticuloendotheliosis

项目摘要

Langerhans Cell Histiocytosis (LCH) is a disease in which tissue destruction is caused by accumulation of histiocytes related to Langerhans cells (LC), the antigen presenting dendritic cells of skin. Although these pathologic LC's (PLC's) are clonal and overexpress p53, the high rate of remission in response to local treatment has led to the consensus that LCH is not a malignancy. LCH lesions can be localized and easily treated, or disseminated and lead to multiorgan failure and death. LC's are motile cells and their trafficking in vivo is tightly regulated. Normal resting LC's express the chemokine receptor CCR6 which directs them to mucocutaneous inflammatory sites where its ligand is secreted. Once LC's ingest antigen and become activated, they down- regulate CCR6 and up-regulate CCR7. This attracts LC's to lymph nodes, the source of CCR7's ligands, where they present antigen to T cells. Our preliminary data demonstrate that despite having characteristics of activated LC's, PLC's show persistent expression of CCR6 explaining, in part, their accumulation at inappropriate tissue sites. The experiments in this proposal are designed to elucidate the pathobiology and pathogenesis of LCH by testing the hypotheses that: (1) Dysregulated expression of chemokines and their receptors may be responsible for the persistence of PLC's in target organs; and (2) Clonal PLC's arise from LC's because of the expression of specific genes. To test these hypotheses, I propose the following specific aims: Specific Aim 1. Test primary LCH tissues for abnormalities in chemokine and chemokine receptor expression. This will be done using custom chip-based hybridization techniques and confirmed by immuno- histochemistry. Correlates will be made to a clinical database.

朗格汉斯细胞组织细胞增生症（LCH）是一种由朗格汉斯细胞（LC）相关组织细胞（皮肤的抗原呈递树突细胞）积聚引起的组织破坏疾病。虽然这些病理性LC（PLC）是克隆性的，并且过表达p53，但局部治疗的高缓解率导致了LCH不是恶性肿瘤的共识。LCH病变可局限于局部，易于治疗，或扩散，导致多器官衰竭和死亡。LC是运动细胞，它们在体内的运输受到严格的调控。正常静息LC表达趋化因子受体CCR 6，其将它们引导至分泌其配体的粘膜皮肤炎性位点。一旦LC摄取抗原并被激活，它们下调CCR 6并上调CCR 7。这将LC吸引到淋巴结，CCR 7配体的来源，在那里它们将抗原呈递给T细胞。我们的初步数据表明，尽管具有活化LC的特征，但PLC显示CCR 6的持续表达，部分解释了它们在不适当的组织部位的积累。在这个建议中的实验被设计来阐明LCH的病理生物学和发病机制，通过测试以下假设：（1）趋化因子及其受体的表达失调可能是导致PLC在靶器官中持续存在的原因;和（2）由于特定基因的表达，LC产生克隆PLC。为了验证这些假设，我提出了以下具体目标：具体目标1。检测原发性LCH组织中趋化因子和趋化因子受体表达的异常。这将使用定制的基于芯片的杂交技术进行，并通过免疫组织化学进行确认。将与临床数据库进行关联。