Cardiovascular Effects of Scutellaria baicelensis

描述(申请人提供)：心血管疾病仍然是全世界主要的死亡原因，许多人在医院外死亡。 导致心脏骤停。尽管氧化剂可能在这一重大事件中起着重要作用 心血管疾病，几乎没有做过什么传统的研究 与非传统抗氧化剂相比，可能在其急性治疗中发挥作用。 在过去的一年里，我们的小组研究了阿司匹林的心脏保护作用 黄芩是一种中草药。我们报道了一份摘录 黄芩对活性氧的抑制作用呈剂量依赖性 减少心肌细胞的死亡。我们特别兴奋的是 黄芩提取快速猝灭活性氧的观察 由线粒体产生的物种。能够快速访问 细胞内部位，如线粒体，并减弱活性氧 物种是一个显著的优势，这是一个可能缺乏的特征 目前正在使用的抗氧化剂。在单独的研究中，我们观察到槲皮素， 一种植物类黄酮，抑制内皮素-1和刺激组织型纤溶酶原 血管内皮细胞中的激活剂。因此，我们假设黄酮类化合物 黄芩的有效成分具有显著的抗氧化潜力，它们 调节血管内皮细胞活性物质的浓度。 心脏病是一种复杂的多因素疾病，具有多种潜在的 原因和风险因素。在缺血性心脏病的发展过程中，部位 最初的损伤是血管内皮细胞。在后期，缺血 心肌细胞的再灌流损伤导致心肌细胞的收缩和细胞功能丧失 死亡。我们建议研究丹参的体外药理作用。 黄芩在两种实验模型中的应用：雏鸡胚胎 心肌细胞和人脐静脉内皮细胞。在建议的 项目中，我们将鉴定黄芩中的有效黄酮类成分和 研究它们在心肌细胞中的抗氧化作用，以及2) 血管内皮细胞中血管活性物质的药理作用。我们会 黄芩提取液及其总黄酮类成分(黄芩素和黄芩素 汉黄素、黄芩黄酮I和黄芩黄酮II)在抗氧化剂 培养心肌细胞，并检测黄芩提取物及其提取物对心肌细胞的影响。 黄酮类改变凝血酶刺激的内皮素-1的浓度，并 血管内皮细胞中的组织型纤溶酶原激活剂。此外, 黄芩的抗氧化活性比较 和西洋参。我们项目的成果将用于开发 黄芩活性成分中潜在的新治疗药物 黄瓜属植物。

DESCRIPTION (provided by applicant): Cardiovascular disease remains a leading cause of death throughout the world, with many dying outside the hospital due to cardiac arrest. Although oxidants may play an important role in this major cardiovascular disease, little has been done to examine what role traditional vs. nontraditional antioxidants may play in its acute treatment. During the past year, our group investigated cardioprotective effects of Scutellaria baicalensis, a Chinese medicinal herb. We reported that an extract of Scutellaria baicalensis dose-dependently attenuated reactive oxygen species in cardiomyocytes and decreased cell death. We were particularly excited to observe that Scutellaria baicalensis extract rapidly quenched reactive oxygen species generated in mitochondria. The ability to gain rapid access to intracellular sites, such as mitochondria, and attenuate reactive oxygen species is a significant advantage, a characteristic that may be lacking in antioxidants currently in use. In separate studies, we observed that quercetin, a plant flavonoid, inhibited endothelin-1 and stimulated tissue plasminogen activator in vascular endothelial cells. Thus, we hypothesize that flavonoids of Scutellaria baicalensis have significant antioxidant potential, and they regulate the concentration of endothelial vasoactive mediators. Heart disease is a complex multifactorial disorder with a variety of underlying causes and risk factors. In the development of ischemic heart disease, the site of initial injury is the vascular endothelium. During later stages, ischemic and reperfusion injury to cardiomyocytes lead to loss of contractility and cell death. We propose to investigate in vitro pharmacological effects of Scutellaria baicalensis in two experimental models: embryonic chick cardiomyocytes, and human umbilical vein endothelial cells. In the proposed project, we will identify active flavonoids of Scutellaria baicalensis and investigate their 1) antioxidant action in cardiomyocytes, and 2) pharmacological effects on vasoactive mediators in endothelial cells. We will test whether Scutellaria baicalensis extract and its flavonoids (baicalein and wogonin, skullcapflavone I, and skullcapflavone II) act as antioxidants in cardiomyocytes, and test whether Scutellaria baicalensis extract and its flavonoids change the concentration of thrombin-stimulated endothelin-1, and tissue plasminogen activator in vascular endothelial cells. In addition, antioxidant activity comparison will be made between Scutellaria baicalensis and American ginseng. The results of our project will be used to develop potential new therapeutic agents from active components of Scutellaria baicalensis.